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首页> 外文期刊>Infection and immunity >Identification and Disruption of Two Discrete Loci Encoding Hyaluronic Acid Capsule Biosynthesis GeneshasA, hasB, and hasC inStreptococcus uberis
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Identification and Disruption of Two Discrete Loci Encoding Hyaluronic Acid Capsule Biosynthesis GeneshasA, hasB, and hasC inStreptococcus uberis

机译：鉴定和编码两个离散的编码透明质酸胶囊生物合成基因的基因座基因shashaA，hasB和hasC在乳房链球菌中

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The hyaluronic acid capsule of Streptococcus uberis has been implicated in conferring resistance to phagocytosis by bovine neutrophils. Construction of a bank of random insertion mutants ofS. uberis (strain 0140J) was achieved using the pGh9::ISS1 mutagenesis system (22). Phenotypic screening of approximately 5,000 clones enabled the isolation of 11 acapsular mutants. Southern hybridization indicated that two mutants carried a lesion within a group of genes similar to those involved in the assembly of the hyaluronic acid capsule found in the group AStreptococcus (GAS) has operon. The DNA sequence flanking the points of insertion confirmed the presence of homologues of GAS hasA and hasB in S. uberis. The DNA sequence flanking the ISS1 insertion in another mutant identified a homologue of hasC inS. uberis. The GAS hasABC operon structure was not conserved in S. uberis, and two discrete loci comprising homologues of either hasAB or hasCwere identified. Disruption of S. uberis hasA orhasC resulted in the complete cessation of hyaluronic acid capsule production. Correspondingly, these mutants were found to have lost their resistance to phagocytosis by bovine neutrophils. The bactericidal action of bovine neutrophils on S. uberis0140J was shown unequivocally to depend upon the capsule status of the bacterium.
机译：乳房链球菌的透明质酸胶囊与牛嗜中性粒细胞的吞噬作用有关。构建 S的随机插入突变体库。使用pGh9 :: IS S1 诱变系统获得了uberis （菌株0140J）（22）。大约5,000个克隆的表型筛选使得能够分离11个荚膜突变体。 Southern杂交表明，两个突变体在一组基因中带有一个损伤，这些基因与A 链球菌（GAS）有组中的透明质酸胶囊组装相关。操纵子。插入点两侧的DNA序列证实了 S中存在GAS hasA 和 hasB 的同源物。厄贝里斯。另一个突变体中IS S1 插入的DNA序列鉴定了 S中 hasC 的同源物。厄贝里斯。 GAS hasABC 操纵子结构在 S中不保守。识别了乳房和/或乳房癌，并鉴定了两个由 hasAB 或 hasC 的同源物组成的离散基因座。 S的破坏。乳房的hasA 或 hasC 导致透明质酸胶囊生产的完全停止。相应地，发现这些突变体失去了对牛嗜中性粒细胞吞噬作用的抗性。牛中性粒细胞对 S的杀菌作用。明确显示出乳房uberis 0140J取决于细菌的荚膜状态。

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