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首页> 外文期刊>Infection and immunity >In Vitro Responsiveness of γδ T Cells fromMycobacterium bovis-Infected Cattle to Mycobacterial Antigens: Predominant Involvement of WC1+Cells
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In Vitro Responsiveness of γδ T Cells fromMycobacterium bovis-Infected Cattle to Mycobacterial Antigens: Predominant Involvement of WC1+Cells

机译：牛分枝杆菌感染牛的γδT细胞对分枝杆菌抗原的体外反应：WC1 +细胞的主要参与。

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It is generally accepted that protective immunity against tuberculosis is generated through the cell-mediated immune (CMI) system, and a greater understanding of such responses is required if better vaccines and diagnostic tests are to be developed. γδ T cells form a major proportion of the peripheral blood mononuclear cells (PBMC) in the ruminant system and, considering data from other species, may have a significant role in CMI responses in bovine tuberculosis. This study compared the in vitro responses of αβ and γδ T cells from Mycobacterium bovis-infected and uninfected cattle. The results showed that, following 24 h of culture of PBMC withM. bovis-derived antigens, the majority of γδ T cells from infected animals became highly activated (upregulation of interleukin-2R), while a lower proportion of the αβ T-cell population showed activation. Similar responses were evident to a lesser degree in uninfected animals. Study of the kinetics of this response showed that γδ T cells remained significantly activated for at least 7 days in culture, while activation of αβ T cells declined during that period. Subsequent analysis revealed that the majority of activated γδ T cells expressed WC1, a 215-kDa surface molecule which is not expressed on human or murine γδ T cells. Furthermore, in comparison with what was found for CD4+ T cells, M. bovis antigen was found to induce strong cellular proliferation but relatively little gamma interferon release by purified WC1+ γδ T cells. Overall, while the role of these cells in protective immunity remains unclear, their highly activated status in response to M. bovis suggests an important role in antimycobacterial immunity, and the ability of γδ T cells to influence other immune cell functions remains to be elucidated, particularly in relation to CMI-based diagnostic tests.
机译：人们普遍认为，通过细胞介导的免疫（CMI）系统可产生针对肺结核的保护性免疫，如果要开发更好的疫苗和诊断测试，则需要对此类反应有更深入的了解。 γδT细胞占反刍动物系统外周血单个核细胞（PBMC）的主要部分，考虑其他物种的数据，它们可能在牛结核病的CMI反应中起重要作用。本研究比较了牛分枝杆菌感染和未感染牛的αβ和γδT细胞的体外反应。结果表明，在用emM培养PBMC 24小时后。牛源的抗原，大部分被感染动物的γδT细胞被高度激活（白介素2R的上调），而较低比例的αβT细胞则被激活。在未感染的动物中，类似的反应在较小程度上是明显的。对这种反应动力学的研究表明，在培养的至少7天中，γδT细胞保持明显活化，而在此期间，αβT细胞的活化下降。随后的分析表明，大多数活化的γδT细胞表达WC1，这是一个215 kDa的表面分子，在人或鼠的γδT细胞上不表达。此外，与CD4 + T细胞的发现相比， M。通过纯化的WC1 + γδT细胞可发现牛visvisem诱导强细胞增殖，但γ干扰素释放相对较少。总体而言，尽管这些细胞在保护性免疫中的作用尚不清楚，但它们对 M的响应处于高度激活状态。 bovis 提示其在抗分枝杆菌免疫中的重要作用，而γδT细胞影响其他免疫细胞功能的能力尚待阐明，特别是在基于CMI的诊断测试中。

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