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首页> 外文期刊>Infection and immunity >Intermediate Subunit of the Gal/GalNAc Lectin of Entamoeba histolytica Is a Member of a Gene Family Containing Multiple CXXC Sequence Motifs
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Intermediate Subunit of the Gal/GalNAc Lectin of Entamoeba histolytica Is a Member of a Gene Family Containing Multiple CXXC Sequence Motifs

机译：溶血性变形杆菌的Gal / GalNAc凝集素的中间亚基是包含多个CXXC序列基序的基因家族的成员。

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摘要

Killing by Entamoeba histolytica requires parasite adherence to host galactose- andN-acetyl-d-galactosamine (Gal/GalNAc)-containing cell surface receptors. A 260-kDa heterodimericE. histolytica Gal/GalNAc lectin composed of heavy (Hgl) and light (Lgl) subunits has been previously described. Here we present the cloning and characterization of Igl, a 150-kDa intermediate subunit of the Gal/GalNAc lectin. Igl, Hgl, and Lgl colocalized on the surface membrane of trophozoites. Two unlinked copies of genes encoding Igl shared 81% amino acid sequence identity (GenBank accession no.AF337950 and AF337951). They encoded cysteine-rich proteins with amino- and carboxy-terminal hydrophobic signal sequences characteristic of glycosylphosphatidylinositol (GPI)-anchored membrane proteins. The igl genes lacked carbohydrate recognition domains but were members of a large family of amebic genes containing CXXC and CXC motifs. These data indicate that Igl is part of the parasite's multimolecular Gal/GalNAc adhesin required for host interaction.
机译：用溶血性巴氏杆菌杀死需要附着寄生物以容纳含有半乳糖和 N -乙酰基-d-半乳糖胺（Gal / GalNAc）的细胞表面受体。 260 kDa异二聚体 E。先前已经描述了由重（Hgl）和轻（Lgl）亚基组成的组织溶菌Gal / GalNAc凝集素。在这里，我们介绍Igl的克隆和特征，Igl是Gal / GalNAc凝集素的150 kDa中间亚基。 Igl，Hgl和Lgl共定位在滋养体的表面膜上。编码Igl的基因的两个未链接的拷贝具有81％的氨基酸序列同一性（GenBank登录号AF337950和AF337951）。他们编码了富含半胱氨酸的蛋白，具有糖基磷脂酰肌醇（GPI）锚定的膜蛋白的氨基和羧基末端疏水信号序列。 igl 基因缺少碳水化合物识别结构域，但是包含CXXC和CXC模体的大量阿米巴酸基因家族的成员。这些数据表明，Igl是寄生虫的宿主相互作用所需的多分子Gal / GalNAc粘附素的一部分。

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