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首页> 外文期刊>Infection and immunity >Induction of Cell-Mediated Immunity to Staphylococcus aureus in the Mouse Mammary Gland by Local Immunization with a Live Attenuated Mutant
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Induction of Cell-Mediated Immunity to Staphylococcus aureus in the Mouse Mammary Gland by Local Immunization with a Live Attenuated Mutant

机译：活体减毒突变体的局部免疫诱导小鼠乳腺中金黄色葡萄球菌的细胞介导的免疫。

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The efficacy of intramammary (Ima) immunization with a live attenuated (la) Staphylococcus aureus mutant to protect the mouse mammary gland from infection has previously been established. The present study was aimed at evaluating whether Ima immunization with la-S. aureus can induce cell-mediated immune responses to the pathogen within the mammary gland. Mice were immunized by Ima route with la-S. aureus, and regional lymph node mononuclear cells were obtained thereafter. A higher expression of the interleukin-2 receptor was found on B and T cells from immunized mice when they were compared with control mice. Immunization with la-S. aureus induced strong proliferative responses to S. aureus. Moreover, significantly increased levels of gamma interferon (IFN-γ) were produced by CD4+ T cells when lymphocytes from immunized mice, but not from control mice, were cultured in the presence of staphylococcal antigens. Moreover, a significant increase in the percentage of IFN-γ-producing CD4+ and CD8+ T cells was observed after S. aureus Ima challenge in immunized mice compared to challenged control mice. Our results demonstrated that Ima immunization with la-S. aureus induced primed lymphocyte populations capable of responding against staphylococcal antigens during in vitro stimulation, as well as during in vivo infection by S. aureus. CD4+ and CD8+ T cells appear to be the main lymphocyte subpopulations involved in this response. It is suggested that IFN-γ production induced by Ima immunization may play a pivotal role in the eradication of intracellular staphylococci.
机译：以前已经使用活的减毒（ la ）金黄色葡萄球菌突变体进行乳腺内（ Ima ）免疫保护小鼠乳腺免受感染的功效。成立。本研究旨在评估 Ima 是否用 la-S免疫。金黄色葡萄球菌可以诱导细胞对乳腺内病原体的免疫应答。通过 Ima 途径用 la-S免疫小鼠。此后获得金黄色葡萄球菌和局部淋巴结单核细胞。将免疫小鼠的B和T细胞与对照小鼠进行比较时，发现白细胞介素2受体的表达更高。用 la-S免疫。金黄色葡萄球菌诱导对 S的强烈增殖反应。金黄色。此外，当在葡萄球菌抗原存在下培养免疫小鼠而非对照小鼠的淋巴细胞时，CD4 + T细胞产生的γ-干扰素（IFN-γ）水平显着增加。此外，在 S后，观察到产生IFN-γ的CD4 + 和CD8 + T细胞的百分比显着增加。与攻击对照组小鼠相比，免疫小鼠的金黄色葡萄球菌Ima攻击。我们的结果表明，使用 la-S进行 Ima 免疫。在体外刺激以及 S体内感染期间，金黄色葡萄球菌诱导的致敏淋巴细胞群体均能对葡萄球菌抗原产生反应。金黄色。 CD4 + 和CD8 + T细胞似乎是参与该反应的主要淋巴细胞亚群。提示 Ima 免疫诱导的IFN-γ产生可能在根除细胞内葡萄球菌中起关键作用。

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