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首页> 外文期刊>Infection and immunity >A DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus Induces Protective Immunity in BALB/c Mice
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A DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus Induces Protective Immunity in BALB/c Mice

机译：编码流产布鲁氏菌铜，锌超氧化物歧化酶的DNA疫苗诱导BALB / c小鼠的保护性免疫。

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摘要

This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.
机译：本研究旨在评估编码流产布鲁氏菌铜，锌超氧化物歧化酶（SOD）的DNA疫苗的免疫原性和保护效果。向BALB / c小鼠肌内注射携带SOD基因的质粒DNA（pcDNA-SOD）引起体液和细胞免疫反应。注射了pcDNA-SOD的动物产生了SOD特异性抗体，该抗体表现出的免疫球蛋白G2a（IgG2a）优于IgG1。此外，DNA疫苗在用重组SOD或粗制 Brucella重新刺激后，会引发T细胞增殖反应，并诱导产生γ干扰素，但不诱导白介素10（IL-10）或IL-4。 蛋白，提示在小鼠中诱导了典型的以T-helper-1为主的免疫反应。 pcDNA-SOD（而不是对照载体）在BALB / c小鼠中诱导了强大的，显着水平的针对 B攻击的保护。流产强毒株2308;保护水平与 B诱导的保护水平相似。流产疫苗菌株RB51。总而言之，这些数据表明pcDNA-SOD是用于未来针对布鲁氏菌病的疫苗接种研究的良好候选者。

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