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首页> 外文期刊>Infection and immunity >Interaction of Mycobacterium avium with Human Monocyte-Derived Dendritic Cells
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Interaction of Mycobacterium avium with Human Monocyte-Derived Dendritic Cells

机译：鸟分枝杆菌与人单核细胞衍生树突状细胞的相互作用

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The mechanism by which mycobacteria elicit class I-restricted T-cell responses remains undefined because these organisms have been shown to reside exclusively within membrane-bound vesicles in macrophages (Mφ), their primary host cells. We studied the interaction of M. avium with dendritic cells (DC) because they are the most potent antigen-presenting cells and are abundant atM. avium infection sites. We observed that both DC and Mφ, generated from human peripheral blood monocytes by short-term culture, internalized M. avium. The onset of programmed cell death and the percentage of apoptotic cells in infected DC and Mφ were comparable. However, following infection, DC secreted significantly larger amounts of interleukin-12, but not interleukin-1β, than infected autologous Mφ. Further analysis of infected cells showed that while phagosomes failed to acidify in bothM. avium-infected DC and Mφ, bacilli grew more slowly in DC. Electron microscopy studies revealed that M. aviumresided within endocytic vacuoles in both cell types. The vacuolar membrane surrounding some bacilli in approximately 10% of the vacuoles in DC possessed several breaks. The importance of this finding will have to be addressed in future studies.
机译：分枝杆菌引发I类限制性T细胞应答的机制仍未确定，因为这些生物已被证明仅驻留在其主要宿主细胞巨噬细胞（Mφ）的膜结合囊泡中。我们研究了 M的相互作用。 avium 具有树突状细胞（DC），因为它们是最有效的抗原呈递细胞，并且在 M处很丰富。禽流感感染部位。我们观察到通过短期培养从人外周血单核细胞产生的DC和Mφ内化了 M。 avium 。在感染的DC和Mφ中，程序性细胞死亡的开始和凋亡细胞的百分比是可比的。但是，感染后，DC分泌的白细胞介素12数量明显高于被感染的自体Mφ，但白细胞介素1β分泌的数量却不多。对感染细胞的进一步分析表明，虽然吞噬体在两个em中均未酸化。鸟感染了DC和Mφ，细菌在DC中的生长较慢。电子显微镜研究表明， M。两种细胞类型中，鸟卵都位于内吞空泡中。在DC中约10％的液泡中围绕一些细菌的液泡膜具有多个断裂。这一发现的重要性必须在未来的研究中加以解决。

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