• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Effects of Gamma Interferon, Interleukin-10, and Transforming Growth Factor β on the Survival of Mycobacterium avium subsp. paratuberculosis in Monocyte-Derived Macrophages from Naturally Infected Cattle
【24h】

Effects of Gamma Interferon, Interleukin-10, and Transforming Growth Factor β on the Survival of Mycobacterium avium subsp. paratuberculosis in Monocyte-Derived Macrophages from Naturally Infected Cattle

机译：γ干扰素，白介素10和转化生长因子β对鸟分枝杆菌亚种存活的影响。自然感染牛单核细胞衍生巨噬细胞中的副结核病

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gamma interferon (IFN-γ) plays a significant role in the control of mycobacterial infections, including Mycobacterium avium subsp. paratuberculosis. However, the contribution of other immunoregulatory cytokines, such as interleukin-10 (IL-10) and transforming growth factor β (TGF-β), in Johne's disease has not been investigated as yet. In this study, we examined the effects of in vivo and in vitro infection with M. avium subsp. paratuberculosis on the production of IFN-γ, IL-10, and TGF-β by peripheral blood mononuclear cells (PBMC). We also examined the effects of exogenous IFN-γ, IL-10, and TGF-β on M. avium subsp. paratuberculosis survival in the cell cultures. PBMC obtained from naturally infected cows, regardless of their disease status, specifically upregulated IL-10 and TGF-β in culture supernatants in response to stimulation with live M. avium subsp. paratuberculosis. Nonstimulated PBMC recovered from subclinically infected animals secreted the lowest levels of TGF-β, but after stimulation with live M. avium subsp. paratuberculosis, TGF-β levels in the culture supernatants increased to levels similar to that produced by PBMC from healthy animals. The numbers of viable M. avium subsp. paratuberculosis recovered from cultures from naturally infected animals were higher than those from healthy cows after in vitro infection with M. avium subsp. paratuberculosis. The addition of exogenous IL-10 and TGF-β to PBMC isolated from healthy cows inhibited the bactericidal activity of these cells as evidenced by the increased number of viable M. avium subsp. paratuberculosis recovered from these cultures compared to cell cultures containing medium alone. These data suggest important immune regulatory roles for IL-10 and TGF-β during infection with M. avium subsp. paratuberculosis that may be directly related to their effects on macrophage activation and killing of M. avium subsp. paratuberculosis.
机译：γ-干扰素（IFN-γ）在控制分枝杆菌感染中起着重要作用，包括鸟分枝杆菌亚种。 肺结核。然而，尚未研究其他免疫调节细胞因子，例如白介素10（IL-10）和转化生长因子β（TGF-β）在约翰内氏病中的作用。在这项研究中，我们检查了 M的体内和体外感染的影响。 avium 子空间肺结核对外周血单个核细胞（PBMC）产生IFN-γ，IL-10和TGF-β的影响。我们还检查了外源性IFN-γ，IL-10和TGF-β对 M的影响。 avium 子空间细胞中副结核的存活率。从自然感染的母牛获得的PBMC，无论其疾病状况如何，都响应活M刺激而特异性上调培养上清液中的IL-10和TGF-β。 avium 子空间肺结核。从亚临床感染的动物中恢复的未刺激的PBMC分泌最低水平的TGF-β，但用活的M刺激后。 avium 子空间在肺结核中，培养上清液中的TGF-β水平升高至与健康动物PBMC产生的水平相似。可行的 M数。 avium 子空间从自然感染动物的培养物中回收的副结核高于在 M体外感染后从健康牛得到的副结核。 avium 子空间肺结核。从健康奶牛分离出的PBMC中添加外源IL-10和TGF-β抑制了这些细胞的杀菌活性，这由存活的M数量增加所证明。 avium 子空间与仅含有培养基的细胞培养物相比，从这些培养物中回收的副结核。这些数据表明在 M感染期间，IL-10和TGF-β具有重要的免疫调节作用。 avium 子空间副结核可能直接与它们对巨噬细胞激活和杀死 M的作用有关。 avium 子空间肺结核。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号