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首页> 外文期刊>Infection and immunity >Passive transfer of poly-(1-6)-beta-glucotriosyl-(1-3)-beta-glucopyranose glucan protection against lethal infection in an animal model of intra-abdominal sepsis.
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Passive transfer of poly-(1-6)-beta-glucotriosyl-(1-3)-beta-glucopyranose glucan protection against lethal infection in an animal model of intra-abdominal sepsis.

机译：在腹部脓毒症动物模型中，聚（1-6）-β-葡萄糖三糖基-（1-3）-β-吡喃葡萄糖葡聚糖的被动转移可防止致命性感染。

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Previous studies have established the efficacy of soluble polymers of poly-(1-6)-beta-glucotriosyl-(1-3)-beta-glucopyranose (PGG) glucan, a biological-response modifier, in protecting against mortality associated with experimentally induced peritonitis in a rat model. PGG glucan-treated animals showed increases in total leukocyte counts and enhanced bacterial clearance from blood. To further explore the mechanisms) by which this agent confers protection, studies were performed to examine whether protection could be transferred from PGG glucan-treated animals to naive recipients via spleen cells (SC), SC lysates, or serum. Passive-transfer experiments indicated that the responsible factor(s) was transferable by whole SC and SC lysates, as well as by peripheral leukocytes or serum from animals treated with PGG glucan. The transferable factor(s) was resistant to pronase and trypsin digestion, was heat stable at 56 or 80 degrees C, and was not removed by NH4SO4 precipitation. The protective effect of PGG glucan was abrogated by treatment with indomethacin, a potent inhibitor of prostaglandin synthesis. Administration of a purified prostaglandin extract from the sera of PGG glucan-treated animals protected against mortality in the peritonitis model. Furthermore, treatment of rats with exogenous synthetic prostaglandin E2 also conferred protection against mortality. These results suggest that the protective effect exhibited by PGG glucan in the rat peritonitis model is mediated, at least in part, by prostaglandins.
机译：先前的研究已经确定了生物反应修饰剂聚（1-6）-β-葡萄糖三糖基-（1-3）-β-吡喃葡萄糖（PGG）葡聚糖的可溶性聚合物在预防与实验诱导相关的死亡率方面的功效大鼠模型中的腹膜炎。经PGG葡聚糖处理的动物显示白细胞总数增加，并且细菌与血液的清除率提高。为了进一步探索该药剂赋予保护作用的机制，进行了研究以检查保护作用是否可以通过脾细胞（SC），SC裂解物或血清从PGG葡聚糖处理过的动物转移至幼稚受体。被动转移实验表明，整个SC和SC裂解物，以及用PGG葡聚糖处理过的动物的外周血白细胞或血清都可以转移责任因子。可转移因子对链霉蛋白酶和胰蛋白酶消化具有抵抗力，在56或80摄氏度下具有热稳定性，并且不会被NH4SO4沉淀除去。通过用吲哚美辛（一种有效的前列腺素合成抑制剂）治疗，可以消除PGG葡聚糖的保护作用。给予经PGG葡聚糖处理的动物血清纯化的前列腺素提取物可预防腹膜炎模型的死亡。此外，用外源性合成前列腺素E2对大鼠的治疗也赋予了针对死亡的保护作用。这些结果表明，PGG葡聚糖在大鼠腹膜炎模型中表现出的保护作用至少部分地由前列腺素介导。

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