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首页> 外文期刊>Infection and immunity >A live (delta aroA) Aeromonas salmonicida vaccine for furunculosis preferentially stimulates T-cell responses relative to B-cell responses in rainbow trout (Oncorhynchus mykiss).
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A live (delta aroA) Aeromonas salmonicida vaccine for furunculosis preferentially stimulates T-cell responses relative to B-cell responses in rainbow trout (Oncorhynchus mykiss).

机译：相对于虹鳟鱼（Oncorhynchus mykiss）的B细胞反应，用于呋喃菌病的活（δaroA）鲑鱼气单胞菌疫苗优先刺激T细胞反应。

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We have previously described (L. M. Vaughan, P. R. Smith, and T. J. Foster, Infect. Immun. 61:2172-2181, 1993) the construction of a kanamycin-resistant aromatic-dependent mutant of Aeromonas salmonicida, the causative agent of furunculosis, and its use as a live vaccine. Here we describe the construction of an unmarked aroA deletion mutant and examine the nature and magnitude of immune responses in rainbow trout (Oncorhynchus mykiss) to this vaccine strain. Good proliferative and antibody responses were elicited by using a range of vaccine doses from 2 x 10(6) to 2 x 10(9) live bacteria per fish, and a clear vaccine dose effect was apparent. A significant positive effect of using live bacteria to prime for lymphocyte proliferation and antibody production was apparent. However, the problem of directly comparing the vaccine doses of live and killed bacterial preparations is discussed, since some replication of live bacteria in vivo is expected. Most importantly, the live vaccine preferentially stimulated enhanced T-cell responsiveness, as evidenced by significantly greater increases in T-cell proliferation than in B-cell proliferation, compared with responses by the respective cell populations from fish given a killed vaccine. The manner in which live vaccines elicit strong cell-mediated immune responses and the relevance to fish vaccine design are discussed.
机译：先前我们已经描述过（LM Vaughan，PR Smith，和TJ Foster，Infect.Immun.61：2172-2181，1993），耐卡那霉素的芳香族依赖性沙门气单胞菌突变体的构建，其是真菌病的病原体，及其用作活疫苗。在这里，我们描述了一个未标记的aroA缺失突变体的构建，并检查了虹鳟鱼（Oncorhynchus mykiss）对这种疫苗株的免疫反应的性质和大小。通过使用每只鱼2 x 10（6）到2 x 10（9）活细菌的疫苗剂量范围，可以引起良好的增殖和抗体反应，并且明显的疫苗剂量效果是显而易见的。使用活细菌引发淋巴细胞增殖和产生抗体具有明显的积极作用。但是，讨论了直接比较活细菌和灭活细菌制剂疫苗剂量的问题，因为预计活细菌会在体内复制。最重要的是，与给予死疫苗的鱼的各个细胞群的反应相比，活疫苗优先刺激增强的T细胞反应性，这可以通过T细胞增殖比B细胞增殖明显更大的增加来证明。讨论了活疫苗引起强烈的细胞介导的免疫反应的方式以及与鱼疫苗设计的相关性。

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