...
首页> 外文期刊>Infection and immunity >Cytokine Profiles for Peripheral Blood Lymphocytes from Patients with Active Pulmonary Tuberculosis and Healthy Household Contacts in Response to the 30-Kilodalton Antigen ofMycobacterium tuberculosis
【24h】

Cytokine Profiles for Peripheral Blood Lymphocytes from Patients with Active Pulmonary Tuberculosis and Healthy Household Contacts in Response to the 30-Kilodalton Antigen ofMycobacterium tuberculosis

机译:活动性肺结核和健康家庭接触者对结核分枝杆菌的30-Kilodalton抗原反应患者外周血淋巴细胞的细胞因子谱

获取原文
           

摘要

Patients with active tuberculosis (TB) have a stronger humoral but a poorer cellular immune response to the secreted 30-kDa antigen (Ag) of Mycobacterium tuberculosis than do healthy household contacts (HHC), who presumably are more protected against disease. The basis for this observation was studied by examining the Th1 (interleukin 2 [IL-2] and gamma interferon [IFN-γ])- and Th2 (IL-10 and IL-4)-type cytokines produced in response to the 30-kDa Ag by peripheral blood mononuclear cells (PBMC) from patients with active pulmonary TB (n = 7) and from HHC who were tuberculin (purified protein derivative) skin test positive (n = 12). Thirty-kilodalton-Ag-stimulated PBMC from TB patients produced significantly lower levels of IFN-γ (none detectable) than did those from HHC (212 ± 73 pg/ml, mean ± standard error) (P < 0.001). Likewise, 30-kDa-Ag-stimulated PBMC from TB patients failed to express IFN-γ mRNA by reverse transcription-PCR, whereas cells from HHC expressed the IFN-γ gene. In contrast, 30-kDa-Ag-stimulated PBMC from TB patients produced significantly higher levels of IL-10 (403 ± 80 pg/ml) than did those from HHC (187 ± 66 pg/ml) (P < 0.013), although cells from both groups expressed the IL-10 gene. IL-2 and IL-4 were not consistently produced, and their genes were not expressed by 30-kDa-Ag-stimulated cells from either TB patients or HHC. After treatment with antituberculous drugs, lymphocytes from four of the seven TB patients proliferated and three of them expressed IFN-γ mRNA in response to the 30-kDa Ag and produced decreased levels of IL-10.
机译:与健康家庭接触者(HHC)相比,活动性肺结核(TB)患者对结核分枝杆菌的分泌的30 kDa抗原(Ag)的体液较强,但细胞免疫反应较弱。保护免受疾病侵害。通过检查Th1(白介素2 [IL-2]和γ干扰素[IFN-γ])和Th2(IL-10和IL-4)型细胞因子对30-活动肺结核( n = 7)和结核菌素(纯化蛋白衍生物)的HHC患者外周血单核细胞(PBMC)的kDa Ag阳性( n = 12)。与HHC相比,来自结核病患者的30 klodaltonton-Ag刺激的PBMC产生的IFN-γ水平显着降低(未检测到)(212±73 pg / ml,平均值±标准误差)( P <0.001)。同样,来自结核病患者的30 kDa-Ag刺激的PBMC不能通过逆转录PCR表达IFN-γmRNA,而HHC的细胞表达IFN-γ基因。相比之下,来自结核病患者的30 kDa-Ag刺激的PBMC产生的IL-10水平(403±80 pg / ml)明显高于HHC(187±66 pg / ml)( P <0.013),尽管两组细胞均表达IL-10基因。 IL-2和IL-4的产生不一致,并且来自TB患者或HHC的30 kDa-Ag刺激的细胞未表达其基因。用抗结核药治疗后,七名结核病患者中有四名的淋巴细胞增生,其中三名表达对30 kDa Ag的IFN-γmRNA表达,并降低了IL-10水平。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号