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首页> 外文期刊>Infection and immunity >Mouse β-Defensin 1 Is a Salt-Sensitive Antimicrobial Peptide Present in Epithelia of the Lung and Urogenital Tract
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Mouse β-Defensin 1 Is a Salt-Sensitive Antimicrobial Peptide Present in Epithelia of the Lung and Urogenital Tract

机译：小鼠β-防御素1是存在于肺和泌尿生殖道上皮细胞中的盐敏感性抗菌肽

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One component of host defense at mucosal surfaces appears to be epithelium-derived peptides with antimicrobial activity called defensins. Human β-defensin 1 (hBD-1) represents the first member of the β-defensin family isolated from humans and has been implicated in the pathogenesis of cystic fibrosis. We describe in this report the isolation and characterization of a murine homolog of hBD-1 called mouse β-defensin 1 (mBD-1). The predicted amino acid sequence shows the hallmark features of other known epithelial β-defensins, including the ordered array of six cysteine residues. Analysis of a genomic clone of mBD-1 revealed two exons separated by a 15-kb intron. By use of fluorescence in situ hybridization, the mBD-1 gene was localized at the proximal portion of chromosome 8, the site where mouse α-defensins are found. Lysates from cells transfected with the mBD-1 cDNA showed antibacterial activity against gram-positive and gram-negative bacteria. mBD-1 transcripts were found in kidney, liver, and female reproductive organ tissues. In the airways, mBD-1 is expressed diffusely throughout the epithelial cells of the large proximal airways with less expression in the small distal airways and no expression in alveolar cells. The present study demonstrates that a β-defensin potentially homologous to human β-defensin 1 is present in the respiratory system and other mucosal surfaces in mice.
机译：粘膜表面宿主防御的一种成分似乎是具有抗微生物活性的上皮衍生肽，称为防御素。人β-防御素1（hBD-1）代表从人体内分离出的β-防御素家族的第一个成员，与囊性纤维化的发病机理有关。我们在这份报告中描述了称为小鼠β-防御素1（mBD-1）的hBD-1鼠同源物的分离和表征。预测的氨基酸序列显示了其他已知的上皮β-防御素的标志性特征，包括六个半胱氨酸残基的有序阵列。对mBD-1的基因组克隆进行分析后发现，两个外显子被15kb的内含子隔开。通过使用荧光原位杂交，mBD-1基因位于第8号染色体的近端，这是发现小鼠α-防御素的位置。用mBD-1 cDNA转染的细胞的裂解物显示出对革兰氏阳性和革兰氏阴性细菌的抗菌活性。在肾脏，肝脏和女性生殖器官组织中发现了mBD-1转录本。在气道中，mBD-1在整个大近端气道的上皮细胞中广泛表达，在小远端气道中的表达较少，在肺泡细胞中无表达。本研究表明，小鼠的呼吸系统和其他粘膜表面存在与人β-防御素1潜在同源的β-防御素。

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