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首页> 外文期刊>Infection and immunity >Characterization of Allelic Variation in theBabesia bovis Merozoite Surface Antigen 1 (MSA-1) Locus and Identification of a Cross-Reactive Inhibition-Sensitive MSA-1 Epitope
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Characterization of Allelic Variation in theBabesia bovis Merozoite Surface Antigen 1 (MSA-1) Locus and Identification of a Cross-Reactive Inhibition-Sensitive MSA-1 Epitope

机译：牛肝菌裂殖子表面抗原1（MSA-1）位点的等位基因变异的表征和交叉反应抑制敏感性MSA-1表位的鉴定。

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The Babesia bovis merozoite surface antigen 1 (MSA-1), a member of the variable merozoite surface antigen (VMSA) family, is an immunodominant glycoprotein which elicits antibodies that inhibit erythrocyte invasion. While antigenic polymorphism is a general feature of vmsa genes, the molecular basis and extent ofmsa-1 sequence polymorphism have not been well characterized. In this study we defined the msa-1 locus in the biologically cloned Mexico Mo7 strain of B. bovis and identified the sequence differences between MSA-1 antigenically dissimilar strains. We then determined whether sequences conserved between distinct msa-1 alleles would induce cross-reactive CD4+ T lymphocytes or inhibitory antibodies. Themsa-1 locus in Mo7 contains a single msa-1 gene flanked by transcribed genes with no sequence homology to members of the VMSA gene family. Argentina B. bovis strains R1A and S2P have msa-1 genes with amino acid sequences that are 98.8% identical to each other, and antibodies against S2P MSA-1 cross-react with native R1A MSA-1. In contrast, identity between the Argentina and Mexico Mo7 msa-1 alleles is only 52%, with no continuous stretch of identity longer than 16 amino acids. Despite limited sequence conservation, antibodies against R1A MSA-1 were able to inhibit invasion of erythrocytes by Mo7 merozoites. The results indicate that inhibition-sensitive epitopes are conserved despite significant sequence divergence between Mexico and Argentina strain alleles and support a conserved functional role for polymorphic MSA-1 in erythrocyte invasion.
机译： Bovissia bovis 裂殖子表面抗原1（MSA-1）是可变裂殖子表面抗原（VMSA）家族的成员，是一种免疫优势糖蛋白，可引发抑制红细胞入侵的抗体。抗原多态性是 vmsa 基因的一个普遍特征，但 msa-1 序列多态性的分子基础和程度尚不十分清楚。在这项研究中，我们在生物克隆的 B墨西哥Mo7菌株中定义了 msa-1 基因座。并鉴定了MSA-1抗原异种菌株之间的序列差异。然后我们确定在不同的 msa-1 等位基因之间保守的序列是否会诱导交叉反应性CD4 + T淋巴细胞或抑制性抗体。 Mo7中的 msa-1 基因座包含一个单一的 msa-1 基因，其侧翼是转录的基因，与VMSA基因家族的成员没有序列同源性。阿根廷 B。 bovis 菌株R1A和S2P具有 msa-1 基因，它们的氨基酸序列彼此相同，为98.8％，并且抗S2P MSA-1的抗体与天然R1A MSA-1发生交叉反应。相反，阿根廷和墨西哥Mo7 msa-1 等位基因之间的同一性仅为52％，没有连续的同一性延伸超过16个氨基酸。尽管序列保守性有限，但针对R1A MSA-1的抗体仍能够抑制Mo7裂殖子对红细胞的入侵。结果表明，尽管在墨西哥和阿根廷菌株等位基因之间存在明显的序列差异，但抑制敏感的抗原决定簇仍然保守，并支持多态性MSA-1在红细胞侵袭中的保守功能。

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