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首页> 外文期刊>Infection and immunity >Immune cell lethality induced by streptococcal pyrogenic exotoxin A and endotoxin.
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Immune cell lethality induced by streptococcal pyrogenic exotoxin A and endotoxin.

机译：链球菌热原性外毒素A和内毒素诱导的免疫细胞致死率。

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Streptococcal pyrogenic exotoxin (SPE) A has many effects on the immune system, including immunolethality, which is characterized by a significant decrease in circulating immune cells as well as depletion of the spleen and lymph nodes prior to death of experimental animals. In this report, characterization of the mechanism of immunolethality has been undertaken. Synergistic induction of immunolethality was observed in vitro when human lymphocytes were treated with both SPE A and lipopolysaccharide (LPS). The same effect was demonstrated in the absence of a mitogenic response with the murine T-cell receptor, as well as in the absence of antigen-presenting cells and their secreted cytokines. The addition of antigen-presenting cells did not significantly affect lethality. SPE A directly interacted with LPS through interaction with ketodeoxyoctonate as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and iodinated exotoxin overlays. This interaction was demonstrated to be important for immunolethality, since simultaneous addition of SPE A and LPS was required, whereas sequential addition of SPE A and LPS did not result in lethality. LPS appeared to be acting, in part, to enhance the cell-binding ability of SPE A, since SPE A could only be detected in A.E7 cell membrane preparations after simultaneous incubation with SPE A and LPS.
机译：链球菌热原性外毒素（SPE）A对免疫系统有许多影响，包括免疫致死性，其特征是循环免疫细胞显着减少以及实验动物死亡前脾脏和淋巴结的消耗。在本报告中，已经对免疫致死性机制进行了表征。当用SPE A和脂多糖（LPS）共同处理人淋巴细胞时，在体外观察到协同免疫致死性。在不存在鼠T细胞受体的促有丝分裂反应以及不存在抗原呈递细胞及其分泌的细胞因子的情况下，证明了相同的效果。抗原呈递细胞的添加并没有显着影响杀伤力。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和碘化外毒素覆盖层表明，SPE A通过与酮氧基辛酸酯的相互作用直接与LPS相互作用。这种相互作用对免疫致死性很重要，因为需要同时添加SPE A和LPS，而顺序添加SPE A和LPS不会导致致死性。 LPS似乎在部分程度上增强了SPE A的细胞结合能力，因为SPE A仅在与SPE A和LPS同时孵育后才能在A.E7细胞膜制剂中检测到。

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