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首页> 外文期刊>Infection and immunity >Differential myelopoietic responsiveness of BALB/c (Itys) and C.D2 (Ityr) mice to lipopolysaccharide administration and Salmonella typhimurium infection.
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Differential myelopoietic responsiveness of BALB/c (Itys) and C.D2 (Ityr) mice to lipopolysaccharide administration and Salmonella typhimurium infection.

机译：BALB / c（Itys）和C.D2（Ityr）小鼠对脂多糖给药和鼠伤寒沙门氏菌感染的差异性骨髓生成反应。

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摘要

Inheritance of the Ityr or the Itys allele of the Ity murine gene confers resistance or increased susceptibility, respectively, to Salmonella typhimurium infection. Recent studies have documented that Ity gene expression may determine net intracellular replication of S. typhimurium by modulating macrophage function. The purpose of this study was to determine if Ity gene expression modulated macrophage stem cell proliferation as well. To detect possible Ity-associated alterations in macrophage stem cell proliferation during endotoxin challenge or S. typhimurium infection, the congenic strain pair BALB/c (Itys) and C.D2-Idh-1, Pep-3 N20F8 (Ityr) were injected intraperitoneally with 25 micrograms of bacterial lipopolysaccharide (LPS) or approximately 10(3) S. typhimurium, and myelopoiesis was evaluated. At 72 h after LPS injection, both BALB/c and C.D2 mice developed comparable degrees of bone marrow hypocellularity and splenomegaly, and cell sizing profiles indicated a normal response to a single injection of LPS in both strains of mice. Although an inhibitor to colony-stimulating factor activity was detected in the sera and plasma of C.D2 mice, the number of myeloid stem cells cultured from the bone marrow and spleen of each mouse strain were comparable. S. typhimurium infection resulted in earlier symptoms, a larger bacterial load, a higher mortality rate, and a greater bone marrow hypocellularity and splenomegaly in BALB/c mice compared with those in C.D2 mice. Despite a dramatic increase in bacterial load, a decrease in both bone marrow and splenic myeloid stem cell numbers was noted in BALB/c mice, while stem cell numbers remained constant in C.D2 mice between days 3 and 5 and increased dramatically at day 7 after infection. These data suggest that BALB/c and C.D2 mice may exhibit a divergent myelopoietic response to S. typhimurium infection. It appears that a paradoxical failure of myelopoiesis in Itys mice during S. typhimurium infection may contribute to the observed increase in mortality.
机译：Ity鼠基因的Ityr或Itys等位基因的遗传分别赋予鼠伤寒沙门氏菌感染抗性或易感性。最近的研究表明，Ity基因表达可能通过调节巨噬细胞功能来确定鼠伤寒沙门氏菌的净细胞内复制。这项研究的目的是确定Ity基因表达是否也能调节巨噬细胞干细胞的增殖。为了检测内毒素激发或鼠伤寒沙门氏菌感染过程中巨噬细胞干细胞增殖中可能与伊替定相关的变化，将同基因菌株对BALB / c（Itys）和C.D2-Idh-1，Pep-3 N20F8（伊特尔）腹膜内注射用25微克细菌脂多糖（LPS）或大约10（3）鼠伤寒沙门氏菌和骨髓生成进行评估。注射LPS后72小时，BALB / c和C.D2小鼠均出现了相当程度的骨髓低细胞性和脾肿大，并且细胞大小分布图表明，在这两种小鼠中，单次注射LPS的反应都是正常的。尽管在C.D2小鼠的血清和血浆中检测到了集落刺激因子活性的抑制剂，但是从每种小鼠品系的骨髓和脾脏培养的骨髓干细胞的数量是可比的。与C.D2小鼠相比，鼠伤寒沙门氏菌感染在BALB / c小鼠中导致症状更早，细菌负荷更大，死亡率更高，骨髓细胞减少和脾肿大更大。尽管细菌载量急剧增加，但在BALB / c小鼠中骨髓和脾髓样干细胞数量均减少，而C.D2小鼠中的干细胞数量在第3天和第5天之间保持不变，并在第7天急剧增加。感染后。这些数据表明，BALB / c和C.D2小鼠对鼠伤寒沙门氏菌感染可能表现出不同的骨髓生成反应。看来鼠伤寒沙门氏菌感染期间Itys小鼠的骨髓生成异常矛盾可能导致观察到的死亡率增加。

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