• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Alterations in protein expression and complement resistance of pathogenic Naegleria amoebae.
【24h】

Alterations in protein expression and complement resistance of pathogenic Naegleria amoebae.

机译：致病性变形虫的蛋白质表达和补体抗性的变化。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Highly pathogenic strains of Naegleria fowleri activate the alternative complement pathway but are resistant to lysis. In contrast, weakly pathogenic and nonpathogenic Naegleria spp. activate the complement pathway and are readily lysed. The present study was undertaken to determine whether surface components on amoebae accounted for resistance to complement lysis. Enzymatic removal of surface components from highly pathogenic N. fowleri with phosphatidylinositol-specific phospholipase C or with endoglycosidase H increased the susceptibility of these amoebae to complement-mediated lysis. Similar treatment of nonpathogenic amoebae had no effect on susceptibility to complement. Tunicamycin treatment of highly and weakly pathogenic N. fowleri increased susceptibility to lysis by complement in a dose-related manner. Tunicamycin treatment did not alter the susceptibility of nonpathogenic amoebae to complement. Proteins of 234 and 47 kDa were detected in supernatant fluid from phosphatidylinositol-specific phospholipase C-treated highly pathogenic amoebae but not in supernatant fluid from phosphatidylinositol-specific phospholipase C-treated weakly pathogenic amoebae. Electrophoretic analysis of iodinated surface proteins of highly pathogenic N. fowleri revealed species of 89, 60, 44, and 28 kDa. Western immunoblots of lysates from surface-iodinated amoebae were stained with biotinylated concanavalin A or biotinylated Ulex europaeus agglutinin I. Surface proteins, identified in highly pathogenic amoebae by iodination, were shown to be glycoproteins by lectin analysis specific for the detection of mannose and fucose residues.
机译：高致病性的Naegleria fowleri菌株可以激活替代补体途径，但对裂解具有抗性。相比之下，病原性弱和非致病性的纳格氏菌属。激活补体途径并易于裂解。进行本研究以确定变形虫上的表面成分是否对补体溶解产生抗性。用磷脂酰肌醇特异性磷脂酶C或糖苷内切酶H酶从高致病性福氏猪笼草表面去除酶成分，增加了这些变形虫对补体介导的裂解的敏感性。非致病性变形虫的相似治疗对补体的敏感性没有影响。衣霉素对高致病性和弱致病性禽流感奈瑟氏菌的治疗以剂量相关的方式增加了补体对细胞溶解的敏感性。衣霉素治疗不会改变非致病性变形虫对补体的敏感性。在磷脂酰肌醇特异性磷脂酶C处理的高致病性变形虫的上清液中检测到234和47 kDa的蛋白质，但在磷脂酰肌醇特异性磷脂酶C处理的弱致病性变形虫的上清液中未检测到。对高致病性福氏猪笼草的碘化表面蛋白进行电泳分析，发现其物种为89、60、44和28 kDa。用生物素化的伴刀豆球蛋白A或生物素化的尤里克欧洲凝集素I对来自表面碘化的变形虫的裂解物的Western免疫印迹进行染色。通过碘化在高致病性变形虫中鉴定出的表面蛋白通过凝集素分析显示为糖蛋白，专门用于检测甘露糖和岩藻糖残基。 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号