Morphological and biochemical study of cytoskeletal changes in cultured cells after extracellular application of Clostridium novyi alpha-toxin.

A Oksche; R Nakov; E Habermann

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Morphological and biochemical study of cytoskeletal changes in cultured cells after extracellular application of Clostridium novyi alpha-toxin.

机译：胞外应用新孢梭菌α-毒素后培养细胞的细胞骨架变化的形态学和生化研究。

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Clostridium novyi alpha-toxin caused retraction and rounding of cultured endothelial cells from porcine pulmonary arteries; nevertheless, the endothelial cells firmly adhered to their supports. F-actin stained with fluorescein-labeled phalloidin was condensed around the nucleus, whereas intermediate filaments and microtubules appeared unchanged. The content of F-actin and myosin was decreased, but that of G-actin or vimentin was not. A predominant role of the microfilament system in C. novyi alpha-toxin cytopathic action is suggested.

机译：诺维梭状芽胞杆菌α毒素引起猪肺动脉内皮细胞的退缩和变圆;然而，内皮细胞牢固地粘附在其支持物上。用荧光素标记的鬼笔环肽染色的F-肌动蛋白在细胞核周围凝结，而中间的细丝和微管则保持不变。 F-肌动蛋白和肌球蛋白的含量降低，而G-肌动蛋白或波形蛋白的含量没有降低。建议微丝系统在诺氏梭菌α-毒素细胞病变作用中的主要作用。

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《Infection and immunity》 |1992年第7期|共5页
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A Oksche; R Nakov; E Habermann;
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中图分类医学免疫学;
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1. Pharmacological and biochemical studies of cytotoxicity of Clostridium novyi type A alpha-toxin. [J] . P Bette, J Frevert, F Mauler, Infection and immunity . 1989,第8期

机译：诺氏梭状芽胞杆菌A型毒素细胞毒性的药理和生化研究。
2. Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation [J] . C.s. Mermelstein, L.m. Amaral, M.i.l. Rebello, Brazilian Journal of Medical and Biological Research . 2003,第8期

机译：细胞外钙离子螯合后培养细胞的细胞形态，细胞骨架蛋白和粘附部位的变化
3. Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation [J] . Mermelstein C.S., Rebello M.I.L., Amaral L.M., Brazilian Journal of Medical and Biological Research . 2003,第8期

机译：细胞外钙离子螯合后培养细胞的细胞形态，细胞骨架蛋白和粘附部位的变化
4. Controlling the biochemical environment of extracellular matrix mimics over time to study and influence cell-matrix interactions for applications in regenerative medicine [C] . Catherine Lambert, Vincent Huynh, Devang Nijsure, World biomaterials congress . 2016

机译：控制细胞外基质模拟物的生化环境，以研究和影响细胞-基质相互作用，用于再生医学
5. Cytoskeletal and motility effects of a photoactivatable GTPase signaling inhibitor in cultured mammalian cells. [D] . Marciszyn, Allison L. 2007

机译：光活化的GTPase信号转导抑制剂在哺乳动物细胞中的细胞骨架和运动效应。
6. Morphological and biochemical study of cytoskeletal changes in cultured cells after extracellular application of Clostridium novyi alpha-toxin. [O] . A Oksche, R Nakov, E Habermann 1992

机译：胞外应用新孢梭菌α-毒素后培养细胞的细胞骨架变化的形态学和生化研究。
7. Degradation of connective tissue matrices by macrophages. III. Morphological and biochemical studies on extracellular, pericellular, and intracellular events in matrix proteolysis by macrophages in culture [O] . 1980

机译：巨噬细胞降解结缔组织基质。三，通过培养中的巨噬细胞对基质蛋白水解中细胞外，细胞周围和细胞内事件进行形态学和生化研究

Morphological and biochemical study of cytoskeletal changes in cultured cells after extracellular application of Clostridium novyi alpha-toxin.

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