• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Utilization of fractionated soluble egg antigens reveals selectively modulated granulomatous and lymphokine responses during murine schistosomiasis mansoni.
【24h】

Utilization of fractionated soluble egg antigens reveals selectively modulated granulomatous and lymphokine responses during murine schistosomiasis mansoni.

机译：利用分离的可溶性卵抗原揭示了在曼氏血吸虫病鼠中选择性调节的肉芽肿和淋巴因子应答。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Worm eggs deposited in the livers and intestines of Schistosoma mansoni-infected mice secrete soluble egg antigens (SEA) and induce T cell-mediated circumoval granulomas. In the present study, we fractionated crude SEA by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tested the fractions for granuloma elicitation and lymphokine production at different stages of the infection. SEA fraction-coupled beads were used to elicit artificial pulmonary granulomas. Acutely infected mice responded with granulomas to seven fractions (less than 21-, 25- to 30-, 32- to 38-, 60- to 66-, 70- to 90-, 93- to 125-, and greater than 200-kDa fractions) of SEA, whereas chronically infected mice responded to four fractions (60- to 66-, 70- to 90-, 93- to 125-, and greater than 200-kDa fractions). In response to both crude and fractionated SEA, granuloma T cells produced high levels of gamma interferon at the preacute (6-week) stage of infection, but production subsequently diminished. Interleukin-2 (IL-2) and IL-4 production peaked at the acute (8-week) stage of infection and concurrently decreased at the chronic (20-week) stage. At the acute stage of the infection, the granulomagenic SEA fractions also elicited IL-2 and IL-4 production; at the chronic stage, IL-2 production and, to a lesser degree, IL-4 production corresponded to SEA fractions that elicited granulomas. Isolated SEA proteins from the 32- to 38-kDa fraction demonstrated differential lymphokine responses: predominant gamma interferon and IL-2 production was elicited by the 32-kDa fraction, whereas the 35- and 38-kDa proteins elicited predominant gamma interferon and IL-4 production. However, all three proteins elicited granuloma formation. The present study reveals changes in granulomatous responses to SEA fractions during the acute and chronic stages of the infection as well as distinct phases of gamma interferon, IL-2, and IL-4 lymphokine production throughout the infection. Based on these results, it is concluded that granuloma formation and IL-2 and IL-4 production are interrelated.
机译：曼氏血吸虫感染的小鼠肝脏和肠道中沉积的蠕虫卵分泌可溶性卵抗原（SEA）并诱导T细胞介导的环周肉芽肿。在本研究中，我们通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对粗制SEA进行分级分离，并测试了感染不同阶段肉芽肿诱发和淋巴因子生成的馏分。 SEA分数耦合的珠子用于引发人工肺肉芽肿。急性感染的小鼠对肉芽肿的反应分为七个部分（少于21-，25-30-，32-38-，60-66-，70-90-，93-125-和大于200- kDa分数）的SEA，而慢性感染的小鼠对四个分数（60-66-，70-90-，93-125和大于200-kDa的分数）有反应。对粗制和分级SEA都有反应，肉芽肿T细胞在感染的急性（6周）阶段产生了高水平的γ干扰素，但随后产量减少。白细胞介素2（IL-2）和IL-4的产生在感染的急性期（8周）达到峰值，而在慢性病程（20周）下降。在感染的急性阶段，肉芽肿性SEA组分也引起IL-2和IL-4的产生。在慢性阶段，IL-2的产生和在较小程度上IL-4的产生对应于诱发肉芽肿的SEA组分。从32 kDa到38 kDa的分离SEA蛋白表现出不同的淋巴因子应答：主要由32 kDa的组分引起γ干扰素和IL-2的产生，而由35 kDa和38 kDa的蛋白引起主要的gamma干扰素和IL-2。 4生产。但是，所有这三种蛋白质均引起肉芽肿形成。本研究揭示了在感染的急性和慢性阶段以及整个感染过程中γ干扰素，IL-2和IL-4淋巴因子产生的不同阶段，肉芽肿对SEA分数的反应的变化。根据这些结果，可以得出结论，肉芽肿的形成与IL-2和IL-4的产生是相互关联的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号