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Specific T-cell recognition of the merozoite proteins rhoptry-associated protein 1 and erythrocyte-binding antigen 1 of Plasmodium falciparum.

机译:恶性疟原虫的裂殖子蛋白变态反应相关蛋白1和红细胞结合抗原1的特异性T细胞识别。

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摘要

The merozoite proteins merozoite surface protein 1 (MSP-1) and rhoptry-associated protein 1 (RAP-1) and synthetic peptides containing sequences of MSP-1, RAP-1, and erythrocyte-binding antigen 1, induced in vitro proliferative responses of lymphocytes collected from Ghanaian blood donors living in an area with a high rate of transmission of malaria. Lymphocytes from a large proportion of the Ghanaian blood donors proliferated in response to the RAP-1 peptide, unlike those of Danish control blood donors, indicating that this sequence contains a malaria-specific T-cell epitope broadly recognized by individuals living in an area with a high transmission rate of malaria. Most of the donor plasma samples tested contained immunoglobulin G (IgG) and IgM antibodies recognizing the merozoite proteins, while only a minority showed high IgG reactivity to the synthetic peptides.
机译:裂殖子蛋白裂殖子表面蛋白1(MSP-1)和Rhoptry相关蛋白1(RAP-1)以及含有MSP-1,RAP-1和红细胞结合抗原1序列的合成肽可诱导体外的增殖反应从加纳献血者那里收集的淋巴细胞生活在疟疾传播率很高的地区。与丹麦对照献血者不同,来自大部分加纳献血者的淋巴细胞响应RAP-1肽而增殖,这表明该序列包含疟疾特异的T细胞表位,该抗原表位广泛存在于居住在该地区的人疟疾的高传播率。测试的大多数供体血浆样品均包含识别裂殖子蛋白的免疫球蛋白G(IgG)和IgM抗体,而只有少数对合成肽表现出高IgG反应性。

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