• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Cellular and humoral immune responses in dogs experimentally and naturally infected with Leishmania infantum.
【24h】

Cellular and humoral immune responses in dogs experimentally and naturally infected with Leishmania infantum.

机译：实验性和自然感染婴儿利什曼原虫的狗的细胞和体液免疫反应。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper we describe a number of immunological parameters for dogs with a chronic Leishmania infantum infection which exhibit patterns of progressive disease or apparent resistance. The outcome of infection was assessed by isolation of parasites, serum antibody titers to Leishmania antigen, and development of clinical signs of leishmaniasis. Our studies show that 3 years after experimental infection, asymptomatic or resistant dogs responded to L. infantum antigen both in lymphocyte proliferation assays in vitro and in delayed-type hypersensitivity reaction, whereas no serum antibodies to parasite antigen were shown. In contrast, symptomatic or susceptible animals failed to respond to parasite antigen in cell-mediated assays both in vitro and in vivo and showed considerably higher serum antibodies to leishmanial antigens. In addition, significantly higher level of interleukin 2 (IL-2) and tumor necrosis factor were found in supernatants from stimulated peripheral mononuclear cells from asymptomatic dogs compared with those from symptomatic and control uninfected dogs. IL-6 production did not vary significantly among the groups studied. Finally, we observed similar results with a group of mixed-breed dogs with natural Leishmania infections also grouped as asymptomatic or symptomatic on the basis of clinical signs of canine visceral leishmaniasis. These results demonstrate that serum antibody titers, antigen-specific proliferative responses, delayed-type hypersensitivity skin reactions, and IL-2 and tumor necrosis factor production by peripheral mononuclear cells can be used as markers of disease progression.
机译：在本文中，我们描述了患有慢性婴儿利什曼原虫感染的狗的许多免疫学参数，这些疾病表现出进行性疾病或表观抵抗力的模式。通过分离寄生虫，针对利什曼原虫抗原的血清抗体滴度和利什曼病的临床体征发展评估感染的结果。我们的研究表明，实验性感染后3年，无症状或耐药的犬在体外淋巴细胞增殖测定和迟发型超敏反应中均对婴儿乳杆菌抗原有反应，而未发现针对寄生虫抗原的血清抗体。相反，有症状或易感动物在体外和体内细胞介导的测定中均无法对寄生虫抗原产生反应，并显示出更高的针对利什曼原虫抗原的血清抗体。此外，与无症状和无症状犬相比，无症状犬受刺激的外周单核细胞上清液中白细胞介素2（IL-2）和肿瘤坏死因子的水平明显更高。在所研究的各组中，IL-6的产生没有显着差异。最终，我们观察到了一组具有天然利什曼原虫感染的混合犬，根据犬内脏利什曼病的临床症状也被分为无症状或有症状的，结果相似。这些结果表明，血清抗体滴度，抗原特异性增殖反应，迟发型超敏性皮肤反应以及外周单个核细胞产生的IL-2和肿瘤坏死因子可以用作疾病进展的标志。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号