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首页> 外文期刊>Infection and immunity >Expression of the L8 lipopolysaccharide determinant increases the sensitivity of Neisseria meningitidis to serum bactericidal activity.
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Expression of the L8 lipopolysaccharide determinant increases the sensitivity of Neisseria meningitidis to serum bactericidal activity.

机译：L8脂多糖决定簇的表达增加了脑膜炎奈瑟氏菌对血清杀菌活性的敏感性。

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摘要

The influence of lipopolysaccharide (LPS) expression on the sensitivity of Neisseria meningitidis to serum bactericidal activity was investigated by using a series of variants that expressed different LPS determinants. For each of two different strains, a series of three LPS variants that expressed L3,7, L8, or both were analyzed. LPS variants were identified and monitored by colony blotting with murine monoclonal antibodies specific for the L8 or the L3,7,9 immunotype determinants. Differences in LPS expression were verified by analysis of proteinase K lysates of whole cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then by silver staining or immunoblotting. The variants were used as test strains in bactericidal assays with human sera and with murine sera and monoclonal antibodies. Expression of the L8 LPS epitope was correlated with increased sensitivity to serum bactericidal activity. The geometric mean bactericidal titers of 12 to 16 sets of pre- and postvaccination sera were determined for each variant. Mean serum titers increased progressively with increased expression of L8 on the target strain. The bactericidal titers of anti-outer membrane protein monoclonal antibodies also increased with increased L8 expression.
机译：通过使用表达不同LPS决定簇的一系列变体，研究了脂多糖（LPS）表达对脑膜炎奈瑟氏菌对血清杀菌活性的敏感性的影响。对于两个不同的菌株中的每一个，分析了表达L3,7，L8或两者的一系列三个LPS变体。通过对L8或L3,7,9免疫型决定簇具有特异性的鼠单克隆抗体通过菌落印迹法鉴定和监测LPS变体。 LPS表达的差异通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，然后通过银染或免疫印迹分析全细胞的蛋白酶K裂解物来验证。所述变体在用人血清以及鼠血清和单克隆抗体的杀菌测定中用作测试菌株。 L8 LPS表位的表达与对血清杀菌活性的敏感性增加相关。对于每种变体，确定了接种前和接种后血清的12至16组血清的几何平均滴度。随着目标菌株中L8表达的增加，平均血清滴度逐渐增加。抗外膜蛋白单克隆抗体的杀菌效价也随着L8表达的增加而增加。

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