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首页> 外文期刊>Infection and immunity >Selective translocation of annexins during intracellular redistribution of Chlamydia trachomatis in HeLa and McCoy cells.
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Selective translocation of annexins during intracellular redistribution of Chlamydia trachomatis in HeLa and McCoy cells.

机译：沙眼衣原体在HeLa和McCoy细胞内的细胞内再分布过程中膜联蛋白的选择性易位。

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When Chlamydia trachomatis elementary bodies enter epithelial cells, they occupy membrane-bound vesicles that aggregate with each other in a calcium-dependent manner but that do not fuse with lysosomes. As members of the annexin family of calcium- and membrane-binding proteins have been implicated in mediating calcium-regulated membrane traffic during endo- and exocytosis, we examined the intracellular localization of certain annexins following invasion of HeLa and McCoy cells by C. trachomatis serovar L2. Immunofluorescence staining with a panel of polyclonal antibodies against five human annexins revealed that annexins III, IV, and V translocate within the cytoplasm to the proximity of intracellular chlamydiae whereas the distribution of annexins I and VI was unaffected. The distinct distribution of annexins I and III was further analyzed by confocal microscopy, which revealed an intimate association between chlamydial aggregates or inclusions and annexin III. Confocal microscopy also confirmed the nonassociation of annexin I with chlamydial aggregates. Depletion of intracellular Ca2+ did not prevent association of annexin III with individual elementary body-containing endosomes but did prevent formation of chlamydial aggregates and translocation of annexin III. Furthermore, chloramphenicol-treated cells also showed association between chlamydial aggregates and annexin III, indicating that the annexins are of host cell origin. These data suggest that certain cytosolic annexins may be involved in the Ca(2+)-dependent aggregation and fusion of chlamydia-containing vesicles. The fact that these Ca(2+)-binding proteins differ in their ability to associate with chlamydia-containing vesicles and inclusions implies that the factors that regulate the interaction of annexin I and annexin III with membrane are different and suggests a selective regulatory mechanism for endosome aggregation and avoiding lysosome fusion during chlamydia infection.
机译：当沙眼衣原体基本体进入上皮细胞时，它们占据膜结合的囊泡，它们以钙依赖性方式彼此聚集，但不会与溶酶体融合。由于膜联蛋白家族的钙结合蛋白和膜结合蛋白成员参与内吞和胞吐过程中介导的钙调节膜运输，我们研究了沙眼衣原体血清型侵染Hela和McCoy细胞后某些膜联蛋白的胞内定位。 L2。用一组针对五种人膜联蛋白的多克隆抗体进行的免疫荧光染色显示，膜联蛋白III，IV和V在细胞质内易位至细胞内衣原体的附近，而膜联蛋白I和VI的分布不受影响。共聚焦显微镜进一步分析了膜联蛋白I和膜联蛋白III的独特分布，揭示了衣原体聚集体或内含物与膜联蛋白III密切相关。共聚焦显微镜也证实膜联蛋白I与衣原体聚集体不相关。胞内Ca2 +的耗竭并不能阻止膜联蛋白III与单个基本含体内体的缔合，但可以防止衣原体聚集体的形成和膜联蛋白III的转运。此外，氯霉素处理的细胞还显示衣原体聚集体和膜联蛋白III之间的关联，表明膜联蛋白是宿主细胞起源的。这些数据表明某些胞质膜联蛋白可能参与含衣原体囊泡的Ca（2+）依赖的聚集和融合。这些Ca（2+）结合蛋白与含衣原体的囊泡和内含物缔合的能力不同，这一事实表明调节膜联蛋白I和膜联蛋白III与膜相互作用的因素不同，这表明选择性的调节机制衣原体感染期间内体的聚集并避免溶酶体融合。

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