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首页> 外文期刊>Infection and immunity >Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus.
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Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus.

机译：牛痘病毒表达的恶性疟原虫富含谷氨酸的蛋白的免疫原性。

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The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.
机译：恶性疟原虫F32的gurp基因已被插入痘苗病毒中，并且该重组病毒被命名为VVG4。通过免疫沉淀分析了在VVG4感染的Vero细胞中gurpp的表达，并揭示了大约220,000Da的初级GLURP产物。在细胞内和培养上清液中均检测到GLURP。为了研究牛痘病毒表达的GLURP的免疫原性，用VVG4免疫小鼠，并分析了血清样品与三种多肽的抗体反应性，该多肽几乎覆盖了整个GLURP分子。这三种多肽以重组形式在大肠杆菌中产生。免疫应答主要针对羧基末端重复区域。小鼠抗GLURP血清通过体外培养的恶性疟原虫的免疫沉淀分析识别出真正的GLURP。这些结果表明痘苗病毒表达的glurp产品可以诱导针对来自血期寄生虫的GLURP的体液免疫反应。

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