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首页> 外文期刊>Infection and immunity >Use of anti-CD4+ hybridoma cells to induce Pneumocystis carinii in mice.
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Use of anti-CD4+ hybridoma cells to induce Pneumocystis carinii in mice.

机译：抗CD4 +杂交瘤细胞在小鼠中诱导卡氏肺孢子虫的应用。

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A reduction of peripheral CD4+ cell levels has been correlated with the onset of Pneumocystis carinii pneumonia in AIDS patients. Most in vivo drug discovery and development for P. carinii have been conducted in corticosteroid-treated rats. There is need for the development of new small animal models with more selective methods of immunosuppression. This study outlines a new mouse model in which specific depletion of the CD4+ T-lymphocyte population was achieved by subcutaneous injection of G.K1.5 hybridoma cells into C3HeB/FeJ mice. A significant reduction in splenic CD4+ cells was maintained over a 10-week period following a single injection of cells. Circulating anti-CD4+ antibody was detected throughout the 10-week period in hybridoma-injected mice, while circulating antibody was undetectable 4 weeks after repeated injection of purified monoclonal antibody. There was no significant increase in the CD8+ cell populations of the hybridoma-injected mice. P. carinii cysts increased in the lungs of CD4+ T-cell-depleted mice, with the number of cysts detected comparable to levels in dexamethasone-treated mice. High levels of cysts were detected when CD4+ cell populations in the spleen remained below 5% and decreased when CD4+ populations increased above the 5% level. In mice whose CD4+ population was not reduced below 5%, there was no significant increase in P. carinii cysts detected. This study presents a new mouse model with specific immunosuppression requiring a minimum of animal manipulation for use in discovery and development of potential new therapeutics for P. carinii pneumonia.
机译：艾滋病患者外周血CD4 +细胞水平的降低与卡氏肺孢子虫肺炎的发作有关。 Carinii的大多数体内药物发现和开发已在皮质类固醇治疗的大鼠中进行。需要开发具有更多选择性免疫抑制方法的新型小动物模型。这项研究概述了一种新的小鼠模型，其中通过将G.K1.5杂交瘤细胞皮下注射到C3HeB / FeJ小鼠中来实现CD4 + T淋巴细胞群的特定消耗。单次注射细胞后的十周内，脾脏CD4 +细胞显着减少。在注射杂交瘤细胞的小鼠中，在整个10周的时间内都检测到了循环抗CD4 +抗体，而在重复注射纯化的单克隆抗体后4周未检测到循环抗体。注射了杂交瘤的小鼠的CD8 +细胞数量没有明显增加。 Carnii囊肿在CD4 + T细胞缺失的小鼠的肺部增加，其囊肿数量与地塞米松治疗小鼠的水平相当。当脾脏中的CD4 +细胞数量保持在5％以下时，检测到高水平的囊肿，而当CD4 +群体中的CD4 +细胞数量增加到5％以上时，囊肿水平降低。在其CD4 +种群没有减少到5％以下的小鼠中，检测到的卡氏疟原虫囊肿没有明显增加。这项研究提出了一种新的小鼠模型，该模型具有特定的免疫抑制作用，需要最少的动物操作才能用于发现和开发卡氏肺炎克雷伯氏菌的潜在新疗法。

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