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首页> 外文期刊>Infection and immunity >Comparison of conjugates composed of lipopolysaccharide from Shigella flexneri type 2a detoxified by two methods and bound to tetanus toxoid.
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Comparison of conjugates composed of lipopolysaccharide from Shigella flexneri type 2a detoxified by two methods and bound to tetanus toxoid.

机译：由两种方法解毒并结合破伤风类毒素的弗氏志贺氏菌2a型脂多糖组成的结合物的比较。

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Shigella flexneri type 2a lipopolysaccharide (LPS) was detoxified with acetic acid (O-SP) or with hydrazine (DeALPS). DeALPS, but not O-SP, retained part of its lipid A. Both gave an identical line of precipitation with typing antiserum by double immunodiffusion, and both had low levels of LPS activity by the Limulus amoebocyte lysate assay. O-SP had an M(r) of approximately 17,000. DeALPS had two components of M(r)s approximately 30,00 (major and approximately 10,000 (minor). Adipic acid hydrazide derivatives of O-SP and DeALPS were conjugated to tetanus toxoid (TT), purified by gel filtration through CL-6B Sepharose, and designated O-SP-TT and DeALPS-TT, respectively. Saccharide (2.5 micrograms) as O-SP, DeALPS, or their conjugates was injected subcutaneously into 5-week-old mice three times 2 weeks apart. The mice were bled before the second injection and 7 days after the second and third. O-SP alone did not elicit immunoglobulin M (IgM) or IgG LPS antibodies. DeALPS elicited low levels of IgM LPS antibodies after the third injection only. Two of three lots of O-SP-TT induced significant levels of IgM LPS antibodies after the third injection. One O-SP-TT lot elicited IgG LPS antibodies after the second injection, and all three lots elicited significant levels of IgG after the third. DeALPS-TT induced low levels of anti-LPS IgM and IgG only after the third injection. The geometric mean antibody titers of both immunoglobulin classes induced by O-SP-TT were higher than those induced by DeALPS-TT. By these criteria, O-SP provided a more immunogenic saccharide than DeALPS for S. flexneri type 2a conjugates.
机译：用乙酸（O-SP）或肼（DeALPS）对弗氏志贺氏菌2a型脂多糖（LPS）进行解毒。 DeALPS，但不是O-SP，保留了其部分脂质A。两者通过双重免疫扩散都产生了与抗血清分型相同的沉淀线，并且通过the变形细胞裂解物测定法均具有低水平的LPS活性。 O-SP的M（r）约为17,000。 DeALPS具有大约30,00（主要和大约10,000（次要）的M（r）的两个成分，将O-SP和DeALPS的己二酸酰肼衍生物与破伤风类毒素（TT）结合，并通过CL-6B的凝胶过滤进行纯化用琼脂糖凝胶分别命名为O-SP-TT和DeALPS-TT，将糖精（2.5微克）分别作为O-SP，DeALPS或它们的结合物皮下注射到5周龄的小鼠中，间隔2周3次。第二次注射前以及第二次和第三次注射后7天出血，仅O-SP不会引发免疫球蛋白M（IgM）或IgG LPS抗体; DeALPS仅在第三次注射后才引起低水平的IgM LPS抗体。第三次注射后，O-SP-TT诱导了显着水平的IgM LPS抗体;第二次注射后，一个O-SP-TT批次引发了IgG LPS抗体，第三次注射后，所有三批次均引发了显着水平的IgG。仅在第三次注射后低水平的抗-LPS IgM和IgG tion。 O-SP-TT诱导的两种免疫球蛋白类别的几何平均抗体滴度均高于DeALPS-TT诱导的。根据这些标准，对于弗氏链球菌2a型结合物，O-SP比DeALPS提供了更多的免疫原性糖。

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