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首页> 外文期刊>Infection and immunity >Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili.
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Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili.

机译：编码人肠毒素大肠杆菌的菌毛定殖因子抗原III（CFA / III）主要菌毛蛋白的基因的测序，并证明CFA / III与IV型菌毛有关。

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摘要

The plasmid-encoded structural gene cofA necessary for the production of the major pilin subunit of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli was identified, and the nucleotide sequence of the gene was determined. cofA consists of 714 nucleotides encoding a 238-amino-acid protein (molecular weight of 25,309). CofA seems to be a precursor of CFA/III pilin, because the first 23 residues of the N-terminal amino acid sequence of the purified CFA/III pili coincided with the deduced amino acid sequence for residues 32 to 54 of CofA. Western blot (immunoblot) analysis of CofA also indicated its processing to form mature pilin in the presence of the downstream region of cofA. These results suggest that the major pilin of CFA/III pili is produced as a precursor form which is posttranslationally modified to the mature pilin and forms morphological pili after cleavage of the Gly-30-Met-31 junction, probably by a protease encoded by an as-yet-unknown gene located downstream of cofA. Interestingly, the N-terminal 30-amino-acid sequence of mature CFA/III shows the highest identity (76.7%) to TcpA pilin of Vibrio cholerae, which is a type IV class B pilin.
机译：鉴定了产生人肠毒素性大肠杆菌的菌毛定殖因子抗原III（CFA / III）的主要菌毛蛋白亚基所必需的质粒编码的结构基因cofA，并确定了该基因的核苷酸序列。 cofA由714个核苷酸组成，编码238个氨基酸的蛋白质（分子量为25,309）。 CofA似乎是CFA / III菌毛蛋白的前体，因为纯化的CFA / III菌毛的N末端氨基酸序列的前23个残基与推导的CofA残基32至54氨基酸序列一致。 CofA的蛋白质印迹（免疫印迹）分析还表明，在cofA下游区域存在的情况下，其加工形成成熟的菌毛蛋白。这些结果表明，CFA / III菌毛的主要菌毛蛋白是作为前体形式产生的，其被翻译后修饰成成熟的菌毛蛋白，并在Gly-30-Met-31连接处裂解后形成形态菌毛，这可能是由一种由Gly-30-Met-31编码的蛋白酶引起的。尚未知的基因位于cofA下游。有趣的是，成熟的CFA / III的N端30个氨基酸序列与霍乱弧菌的TcpA菌毛蛋白（IV型B类菌毛蛋白）具有最高的同一性（76.7％）。

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