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首页> 外文期刊>Infection and immunity >Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli.
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Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli.

机译：用在大肠杆菌中合成基因表达的肉毒梭菌神经毒素血清型A重组片段进行保护性疫苗接种。

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摘要

A completely synthetic gene encoding fragment C, a approximately 50-kDa fragment, of botulinum neurotoxin serotype A was constructed from oligonucleotides. The gene was expressed in Escherichia coli, and full-sized product was produced as judged by Western blot (immunoblot) analysis. Crude extracts of E. coli expressing the gene were used to vaccinate mice and evaluate their survival against challenge with active toxin. Mice given three subcutaneous vaccinations were protected against an intraperitoneal administration of 10(6) 50% lethal doses (ID50) of serotype A toxin. The same mice survived when challenged with 3 LD50 of botulinum toxin serotype E but died when challenged with 10 LD50 of serotype E or 3 LD50 of serotype B. Purified fragment C was compared with the botulinum toxoid vaccine in a vaccination and challenge study. Fragment C was as efficacious in protecting against challenge with active botulinum neurotoxin serotype A as the toxoid vaccine. This recombinant protein product has many properties that make it a good candidate for human use to protect against botulinum toxin.
机译：由寡核苷酸构建了编码肉毒杆菌神经毒素血清型A的片段C（约50kDa的片段）的完全合成的基因。该基因在大肠杆菌中表达，并且通过蛋白质印迹（免疫印迹）分析判断产生了全尺寸产物。表达该基因的大肠杆菌粗提物用于给小鼠接种疫苗，并评估其抵抗活性毒素攻击的存活率。给予三种皮下疫苗接种的小鼠被保护免于腹膜内施用10（6）50％致死剂量（ID50）的血清型A毒素。同一只小鼠在接受3 LD50的肉毒杆菌毒素E型攻击时存活，但在受到10份E型的LD50或B血清型3 LD50攻击时死亡。在疫苗接种和攻击研究中，将纯化的片段C与肉毒杆菌类毒素疫苗进行了比较。片段C与类毒素疫苗一样，能有效抵抗活性肉毒杆菌神经毒素血清型A的攻击。这种重组蛋白产品具有许多特性，使其非常适合人类使用以预防肉毒杆菌毒素。

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