An oligomer of the major outer membrane protein of Chlamydia psittaci is recognized by monoclonal antibodies which protect mice from abortion.

C de Sa; A Souriau; F Bernard; J Salinas; A Rodolakis

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An oligomer of the major outer membrane protein of Chlamydia psittaci is recognized by monoclonal antibodies which protect mice from abortion.

机译：鹦鹉热衣原体主要外膜蛋白的寡聚体被保护小鼠免受流产的单克隆抗体识别。

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Monoclonal antibodies (MAbs) were generated against an ovine abortive strain of Chlamydia psittaci. A plaque reduction assay was used to select 19 neutralizing antibodies which appeared to be heterogeneous in isotype, specificity, and recognized proteins. Different neutralizing MAbs were tested for their protective abilities against abortion in a pregnant-mouse model. All of the protective MAbs selected had the same isotype, were serotype 1 specific, and recognized a protein of about 110 kDa by immunoblotting. The recognized epitopes were resistant to sodium dodecyl sulfate and reducing agents, but all of them were heat sensitive. The protein was able to form disulfide-linked polymers. Immunological cross-reaction studies with rabbit sera showed a link between the 110-kDa protein and the major outer membrane protein (MOMP). The 110-kDa protein was purified by immunoaffinity and shown to be dissociated after heating into MOMP by silver staining and immunoblotting. These results show homogeneity among protective MAbs directed to heat-sensitive epitopes located on an oligomer of the MOMP of C. psittaci.

机译：产生针对鹦鹉热衣原体的绵羊流产菌株的单克隆抗体（MAb）。噬斑减少试验用于选择19种中和抗体，这些抗体在同种型，特异性和公认的蛋白质上似乎是异质的。测试了不同的中和单克隆抗体在妊娠小鼠模型中的抗流产保护能力。所选的所有保护性单克隆抗体均具有相同的同种型，具有血清型1特异性，并通过免疫印迹识别约110 kDa的蛋白质。公认的表位对十二烷基硫酸钠和还原剂具有抗性，但是它们都对热敏感。该蛋白质能够形成二硫键连接的聚合物。兔血清的免疫学交叉反应研究表明，110 kDa蛋白与主要外膜蛋白（MOMP）之间存在联系。 110-kDa蛋白通过免疫亲和纯化，并通过银染和免疫印迹显示加热为MOMP后显示解离。这些结果表明，针对热敏抗原决定簇的热敏抗原决定簇的保护性单克隆抗体之间具有均质性。

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《Infection and immunity》 |1995年第12期|共5页
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C de Sa; A Souriau; F Bernard; J Salinas; A Rodolakis;
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1. An oligomer of the major outer membrane protein of Chlamydia psittaci is recognized by monoclonal antibodies which protect mice from abortion. [J] . C de Sa, A Souriau, F Bernard, Infection and immunity . 1995,第12期

机译：鹦鹉热衣原体主要外膜蛋白的寡聚体被保护小鼠免受流产的单克隆抗体识别。
2. Electrophoretic analysis of the major outer membrane protein of Chlamydia psittaci reveals multimers which are recognized by protective monoclonal antibodies. [J] . M C McCafferty, A J Herring, A A Andersen, Infection and immunity . 1995,第6期

机译：对鹦鹉热衣原体的主要外膜蛋白进行电泳分析，揭示了被保护性单克隆抗体识别的多聚体。
3. Electrophoretic analysis of the major outer membrane protein of Chlamydia psittaci reveals multimers which are recognized by protective monoclonal antibodies. [J] . M C McCafferty, A J Herring, A A Andersen, Infection and immunity . 1995,第6期

机译：对鹦鹉热衣原体的主要外膜蛋白进行电泳分析，揭示了被保护性单克隆抗体识别的多聚体。
4. Activation of antigen presenting cells and induction of mucosal antibodies by Chlamydia trachomatis outer membrane peptide encapsulated within PLA-PEG nanoparticles [C] . Saurabh Dixit, Murtada Taha, Shree R. Singh, Annual NSTI nanotechnology conference and expo;TechConnect world innovation conference expo . 2014

机译：包裹在PLA-PEG纳米颗粒内的沙眼衣原体外膜肽激活抗原呈递细胞并诱导粘膜抗体
5. Expression of the Chlamydia psittaci major outer membrane protein in Escherichia coli: A model for translocation and vaccine development. [D] . Dascher, Christopher Carter. 1994

机译：大肠杆菌衣原体主要外膜蛋白在大肠杆菌中的表达：易位和疫苗开发的模型。
6. An oligomer of the major outer membrane protein of Chlamydia psittaci is recognized by monoclonal antibodies which protect mice from abortion. [O] . C de Sa, A Souriau, F Bernard, 1995

机译：鹦鹉热衣原体主要外膜蛋白的寡聚体被保护小鼠免受流产的单克隆抗体识别。
7. An oligomer of the major outer membrane protein of Chlamydia psittaci is recognized by monoclonal antibodies which protect mice from abortion [O] . C de Sa, A Souriau, F Bernard, 1995

机译：衣原体Psittaci的主要外膜蛋白的低聚物被单克隆抗体识别，所述单克隆抗体保护小鼠堕胎

An oligomer of the major outer membrane protein of Chlamydia psittaci is recognized by monoclonal antibodies which protect mice from abortion.

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