• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Antibody response that protects against disseminated candidiasis.
【24h】

Antibody response that protects against disseminated candidiasis.

机译：防止传播念珠菌病的抗体反应。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We previously showed that surface mannans of Candida albicans function as adhesins during yeast cell attachment to mouse splenic marginal zone macrophages. The mannan adhesin fraction was encapsulated into liposomes and used to vaccinate mice over a 5- to 6-week period. Circulating agglutinins specific for the fraction correlated with increased resistance to disseminated candidiasis. Antiserum from vaccinated animals protected naive BALB/cByJ mice against C. albicans serotype A and B strains and Candida tropicalis. Antiserum also protected SCID mice against disseminated disease. The serum protective ability was stable at 56 degrees C, but this ability was adsorbed by C. albicans cells. The antiserum was divided into three fractions after separation by high-performance liquid chromatography. One fraction contained all of the agglutinin activity and transferred resistance to naive mice. A second fraction also transferred resistance. Two monoclonal antibodies (MAbs) specific for candidal surface determinants were obtained. MAb B6.1 is specific for a mannan epitope in the adhesin fraction, and MAb B6 is specific for a different epitope in the fraction. Both MAbs are immunoglobulin M, and both strongly agglutinate candidal cells, but only MAb B6.1 protected both normal and SCID mice against disseminated candidiasis. In one experiment, 10 normal mice were given MAb B6.1 and challenged with yeast cells. Six mice survived the 67-day observation period; 4 of the survivors were cured as evidenced by the lack of CFU in the kidney and spleen. Our studies show that antibodies against certain cell surface antigens of C. albicans help the host resist disseminated candidiasis.
机译：我们以前显示白色念珠菌的表面甘露聚糖在酵母细胞附着到小鼠脾脏边缘区巨噬细胞的过程中充当粘附素。将甘露聚糖粘附素级分封装到脂质体中，并用于在5至6周内为小鼠接种疫苗。特定级分的循环凝集素与对弥散性念珠菌病的抵抗力增强相关。接种疫苗的动物的抗血清可以保护幼稚的BALB / cByJ小鼠免受白色念珠菌血清A和B株和热带念珠菌的侵害。抗血清还保护SCID小鼠免于传播疾病。血清保护能力在56摄氏度下稳定，但这种能力被白色念珠菌细胞吸收。通过高效液相色谱分离抗血清后，将其分为三部分。一小部分包含所有凝集素活性并将抗性转移至幼稚小鼠。第二部分也转移了阻力。获得了两个对候选表面决定簇具有特异性的单克隆抗体（MAb）。 MAb B6.1对粘附素组分中的甘露聚糖表位具有特异性，而MAb B6对组分中的不同表位具有特异性。两种MAb都是免疫球蛋白M，并且都强烈凝集了念珠菌细胞，但是只有MAb B6.1保护了正常小鼠和SCID小鼠免于传播念珠菌病。在一个实验中，向10只正常小鼠给予MAb B6.1并用酵母细胞攻击。六只小鼠在67天的观察期内存活下来;肾脏和脾脏中缺乏CFU证明了其中4名幸存者已治愈。我们的研究表明，针对白色念珠菌某些细胞表面抗原的抗体可帮助宿主抵抗传播的念珠菌病。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号