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首页> 外文期刊>Infection and immunity >Contribution of the major and minor subunits to fimbria-mediated adherence of Haemophilus influenzae to human epithelial cells and erythrocytes.
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Contribution of the major and minor subunits to fimbria-mediated adherence of Haemophilus influenzae to human epithelial cells and erythrocytes.

机译：主要和次要亚基对菌毛介导的流感嗜血杆菌对人上皮细胞和红细胞粘附的贡献。

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摘要

Fimbriae are colonization factors of the human pathogen Haemophilus influenzae in that they mediate bacterial adherence to human eukaryotic cells. The contribution of the major (HifA) and putative minor (HifD and HifE) subunits of H. influenzae fimbriae to fimbria-specific adherence was studied by using mutants that were inactivated in distinct fimbrial genes. Both the major and minor subunits were required for adherence of H. influenzae to oropharyngeal epithelial cells and human erythrocytes carrying the AnWj antigen. Cloning of defined H. influenzae fimbrial genes in an Escherichia coli strain with type 1 fimbriae yielded recombinants expressing high amounts of HifA-containing H. influenzae fimbriae either with or without coexpression of both H. influenzae minor subunits. Both clones exhibited the specific adherence properties of H. influenzae fimbriae, implying that the minor H. influenzae subunits are dispensable for adherence and that the adhesive domain resides in the major subunit, HifA. In H. influenzae itself, the minor subunits probably affect adherence by raising the number of fimbriae above the minimal level required to establish adherence.
机译：菌毛是人类病原体流感嗜血杆菌的定居因子，因为它们介导细菌对人类真核细胞的粘附。通过使用在独特的纤维基因中失活的突变体，研究了流感嗜血杆菌菌毛的主要（HifA）和假定的次要（HifD和HifE）亚基对菌毛特异性粘附的贡献。流感嗜血杆菌对携带AnWj抗原的口咽上皮细胞和人红细胞的粘附都需要主要和次要亚基。克隆具有1型菌毛的大肠杆菌菌株中确定的流感嗜血菌纤维基因，可产生表达量高的含HifA流感嗜血菌菌丝的重组体，无论是否同时表达两个流感嗜血菌小亚基。这两个克隆均显示出流感嗜血杆菌菌毛的特定粘附特性，这意味着次要的流感嗜血菌亚基可用于粘附，而粘附域位于主要的亚基HifA中。在流感嗜血杆菌本身中，次要亚基可能通过将菌毛数量提高至建立依从性所需的最低水平以上来影响依从性。

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