• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Evidence of recombination and an antigenically diverse immunoglobulin A1 protease among strains of Streptococcus pneumoniae.
【24h】

Evidence of recombination and an antigenically diverse immunoglobulin A1 protease among strains of Streptococcus pneumoniae.

机译：肺炎链球菌菌株之间重组和抗原性多样的免疫球蛋白A1蛋白酶的证据。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The genetic relationships among 114 isolates of Streptococcus pneumoniae representing mainly nine serotypes that frequently cause severe childhood disease in Northern Europe were examined by use of multilocus enzyme electrophoresis. A comparison was made of the corresponding antigenic variations of excreted immunoglobulin A1 (IgA1) proteases detected by enzyme neutralization assays. Allelic variation at 13 gene loci among 70 electrophoretic types disclosed a comparatively low mean genetic diversity per locus (H = 0.319). In contrast, IgA1 proteases showed extensive antigenic diversity as 17 different inhibition types were distinguished. A lack of overall clonality was apparent from the linkage equilibrium of alleles harbored by 28 isolates chosen to represent the genetic diversity of the study population. However, certain clones, such as those marked by identical electrophoretic type, serotype, and IgA1 protease type, persisted for a sufficiently long time to enable clonal spread between distant geographic areas. Among clonally related isolates, examples illustrating a shift of capsular serotype or IgA1 protease type supported the view that recombination occurs in vivo in corresponding genes. In conclusion, over time, horizontal genetic exchange appears to be sufficiently frequent to disrupt the clonal structure otherwise generated by binary fission in natural populations of S. pneumoniae. The clonal instability combined with considerable antigenic heterogeneity renders the pneumococcal IgA1 protease less attractive as a potential component of future vaccines.
机译：使用多基因座酶电泳技术检查了114株肺炎链球菌之间的遗传关系，这些菌株主要代表9种血清型，经常导致北欧严重的儿童期疾病。比较了通过酶中和测定法检测到的排泄的免疫球蛋白A1（IgA1）蛋白酶的相应抗原变异。 70个电泳类型中13个基因位点的等位基因变异显示每个位点的平均遗传多样性相对较低（H = 0.319）。相反，由于区分了17种不同的抑制类型，IgA1蛋白酶显示出广泛的抗原多样性。从被选择用来代表研究人群遗传多样性的28个分离株所藏的等位基因的连锁平衡中，明显缺乏总体克隆性。但是，某些克隆（例如以相同的电泳类型，血清型和IgA1蛋白酶类型标记的克隆）会持续足够长的时间，以使克隆能够在遥远的地理区域之间传播。在克隆相关的分离物中，举例说明荚膜血清型或IgA1蛋白酶类型转移的实例支持以下观点：重组发生在相应基因的体内。总之，随着时间的流逝，水平的遗传交换似乎足够频繁地破坏肺炎链球菌自然种群中原本由二元裂变产生的克隆结构。克隆的不稳定性与相当大的抗原异质性相结合，使得肺炎球菌IgA1蛋白酶作为未来疫苗的潜在成分吸引力降低。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号