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首页> 外文期刊>Infection and immunity >Differential effects of the streptococcal fibronectin-binding protein, FBP54, on adhesion of group A streptococci to human buccal cells and HEp-2 tissue culture cells.
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Differential effects of the streptococcal fibronectin-binding protein, FBP54, on adhesion of group A streptococci to human buccal cells and HEp-2 tissue culture cells.

机译：链球菌纤连蛋白结合蛋白FBP54对A组链球菌对人颊细胞和HEp-2组织培养细胞的粘附的差异作用。

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We have previously demonstrated that fibronectin mediates streptococcal adhesion to host cells and that streptococci interact primarily with the N-terminal domain of fibronectin. FBP54 is a 54-kDa protein from group A streptococci that binds fibronectin. In this report, we show that the N-terminal domain of fibronectin reacts with FBP54 and preferentially blocks streptococcal adhesion to buccal epithelial cells. FBP54 blocked adhesion to human buccal epithelial cells by 80% in a dose-related fashion. In contrast, FBP54 had little effect on adhesion of group A streptococci to HEp-2 tissue culture cells. The fibronectin-binding domain of FBP54 has been localized to the first 89 N-terminal residues of the protein. Experiments using affinity-purified antibodies to this region indicated that the N terminus of FBP54 is exposed on the surface of streptococci in a manner that can interact with immobilized receptors. Analysis of sera from patients with post-streptococcal glomerulonephritis and acute rheumatic fever indicated that FBP54 is expressed in vivo and is immunogenic in the human host. These data indicate that FBP54 is a streptococcal adhesin that is expressed in the human host and that preferentially mediates adhesion to certain types of human cells.
机译：我们以前已经证明纤连蛋白介导链球菌对宿主细胞的粘附，而链球菌主要与纤连蛋白的N末端域相互作用。 FBP54是来自A组链球菌的54 kDa蛋白，与纤连蛋白结合。在此报告中，我们表明纤连蛋白的N末端结构域与FBP54反应，并优先阻断链球菌对颊上皮细胞的粘附。 FBP54以剂量相关的方式阻止了80％的人颊上皮细胞粘附。相反，FBP54对A组链球菌对HEp-2组织培养细胞的粘附作用很小。 FBP54的纤连蛋白结合域已定位到该蛋白质的前89个N末端残基。使用针对该区域的亲和纯化抗体的实验表明，FBP54的N末端以可以与固定受体相互作用的方式暴露于链球菌表面。对链球菌后肾小球肾炎和急性风湿热患者的血清分析表明，FBP54在体内表达，在人宿主中具有免疫原性。这些数据表明FBP54是一种链球菌粘附素，在人宿主中表达，并优先介导对某些类型人细胞的粘附。

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