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首页> 外文期刊>Infection and immunity >Variation of Brucella abortus 2308 infection in BALB/c mice induced by prior vaccination with salt-extractable periplasmic proteins from Brucella abortus 19.
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Variation of Brucella abortus 2308 infection in BALB/c mice induced by prior vaccination with salt-extractable periplasmic proteins from Brucella abortus 19.

机译：事先用来自流产布鲁氏菌的盐可提取的周质蛋白接种疫苗诱导的BALB / c小鼠中流产布鲁氏菌2308感染的变化。

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The study compared the immune and protective responses induced in BALB/c mice vaccinated with six salt-extractable periplasmic protein fractions (Brucella cell surface proteins [BCSP]) of Brucella abortus 19 and later challenge exposed with B. abortus 2308. BCSP70 was precipitated with ammonium sulfate at 70% saturation, and BCSP100 was precipitated with ammonium sulfate at 100% saturation by use of supernatant fluid of BCSP70 that had been precipitated with 70% ammonium sulfate. Four subfractions were separated from BCSP100 by anion-exchange high-performance liquid chromatography (HPLC). Monophosphoryl lipid A (MPL) from Salmonella typhimurium Re mutant strain was used as a potential immune response modifier in some vaccines. Reduced or increased numbers of CFU and increased spleen size in the principal groups of mice relative to that of the nonvaccinated control group were considered protectiveness or virulence (survival) criteria. Results indicated that vaccines prepared from BCSP70 and BCSP100 were moderately protective and immunogenic. The subfractions designated BCSP100-A through BCSP100-D purified by anion-exchange HPLC were not protective when MPL was not used as an immune response modifier. However, two subfractions were associated with significant (P < 0.05) increases in CFU per spleen and splenomegaly in vaccinated mice compared with those in nonvaccinated challenge-exposed mice. MPL enhanced protection or was neutral when used with BCSP70, BCSP100, BCSP100-C, and BCSP100-D. Serologic results of an enzyme-linked immunosorbent assay indicated that MPL modulated the immunoglobulin G responses induced by BCSP70, BCSP100, and subfraction BCSP100-B vaccines only. The overall results suggest that certain proteinaceous periplasmic fractions might serve as virulence or survival factors in B. abortus infections.
机译：该研究比较了用六种盐可提取的周质布鲁氏菌19的可盐提取的周质蛋白组分（布鲁氏菌细胞表面蛋白[BCSP]）接种的BALB / c小鼠诱导的免疫和保护反应，随后用流产布鲁氏菌2308暴露。饱和度为70％的硫酸铵，然后使用已用70％硫酸铵沉淀的BCSP70上清液，以100％饱和度的硫酸铵沉淀BCSP100。通过阴离子交换高效液相色谱（HPLC）从BCSP100分离出四个亚级分。鼠伤寒沙门氏菌Re突变株的单磷酰脂质A（MPL）在某些疫苗中用作潜在的免疫应答修饰剂。相对于未接种疫苗的对照组，主要组小鼠的CFU数量减少或增加以及脾脏大小增加被认为是保护性或毒力（存活）标准。结果表明，从BCSP70和BCSP100制备的疫苗具有中等保护性和免疫原性。当未将MPL用作免疫应答修饰剂时，通过阴离子交换HPLC纯化的命名为BCSP100-A至BCSP100-D的亚组分没有保护性。但是，与未接种挑战的小鼠相比，接种疫苗的小鼠的两个脾脏与脾脏和脾肿大的CFU显着增加（P <0.05）。当与BCSP70，BCSP100，BCSP100-C和BCSP100-D一起使用时，MPL可以增强保护或保持中立。酶联免疫吸附测定的血清学结果表明，MPL仅调节BCSP70，BCSP100和亚组分BCSP100-B疫苗诱导的免疫球蛋白G反应。总体结果表明，某些蛋白质周质级分可能在流产双歧杆菌感染中用作毒力或存活因子。

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