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首页> 外文期刊>Infection and immunity >Neisserial porins may provide critical second signals to polysaccharide-activated murine B cells for induction of immunoglobulin secretion.
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Neisserial porins may provide critical second signals to polysaccharide-activated murine B cells for induction of immunoglobulin secretion.

机译：奈瑟氏菌孔蛋白可向多糖激活的鼠B细胞提供关键的第二信号，以诱导免疫球蛋白的分泌。

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Resting B cells stimulated with dextran-conjugated anti-immunoglobulin D (anti-IgD) antibodies (anti-Ig-dex), a model for B-cell activation in response to polysaccharide antigens, proliferate but secrete little if any Ig, unless additional stimuli are present. In order to elucidate the parameters which costimulate T-cell-independent antipolysaccharide antibody responses during bacterial infections, we tested the capacities of highly purified porin proteins from Neisseria meningitidis and Neisseria gonorrhoeae to augment in vitro proliferation and induce Ig secretion by anti-Ig-dex-activated B cells. Resting B cells, from lipopolysaccharide (LPS)-nonresponsive C3H/HeJ mice, proliferated and secreted IgM in response to each of three distinct porins acting alone. Further, porins, even at concentrations that were minimally inductive when acting alone, were strongly synergistic with anti-Ig-dex for proliferation and Ig secretion. Similar synergistic effects of porins with CD40-ligand were also observed. These effects of porins were shown to occur directly at the level of the B cell. The predominant Ig isotype elicited in response to porins plus anti-Ig-dex or CD40-ligand was IgM (>97%), with the remainder comprising IgG. Surprisingly, picogram-per-milliliter amounts of neisserial LPS were also found to be highly synergistic with anti-Ig-dex for induction of IgM secretion by LPS-responsive C3H/HeN, but not C3H/HeJ, B cells. Thus, these data suggest that porins, as well as LPS, may provide critical second signals for T-cell-independent induction of polysaccharide-specific Ig in response to neisserial and other gram-negative porin-expressing bacterial pathogens, without a requirement for the participation of non-B cell types. These data may also help to explain the potent immunopotentiating effects of porins for polysaccharide-specific, as well as protein-specific, humoral responses in vivo.
机译：用葡聚糖偶联的抗免疫球蛋白D（anti-IgD）抗体（anti-Ig-dex）刺激的静止B细胞增殖，该B细胞响应多糖抗原的活化模型可以增殖，但分泌的Ig很少，除非有其他刺激。存在。为了阐明在细菌感染过程中共同刺激T细胞非依赖性抗多糖抗体反应的参数，我们测试了脑膜炎奈瑟氏球菌和淋病奈瑟氏球菌的高纯度孔蛋白增强体外增殖并通过抗Ig-dex诱导Ig分泌的能力。激活的B细胞。来自对脂多糖（LPS）无反应的C3H / HeJ小鼠的静止B细胞，分别响应三种不同孔蛋白的单独作用而增殖和分泌IgM。此外，孔蛋白即使单独作用时具有最低诱导浓度，也能与抗Ig-dex强烈协同促进增殖和Ig分泌。还观察到孔蛋白与CD40-配体的类似的协同作用。已显示孔蛋白的这些作用直接发生在B细胞的水平。响应孔蛋白加抗-Ig-dex或CD40-配体而引起的主要Ig同种型是IgM（> 97％），其余包括IgG。出人意料的是，还发现每毫升皮脂级的脂多糖与抗Ig-dex高度协同，可诱导LPS反应性C3H / HeN诱导IgM分泌，但对C3H / HeJ，B细胞却无诱导作用。因此，这些数据表明，孔蛋白以及LPS可能为响应于奈瑟氏菌和其他表达革兰氏阴性的细菌表达细菌病原体而对T细胞非依赖性地诱导多糖特异性Ig提供关键的第二信号，而无需非B细胞类型的参与。这些数据也可能有助于解释孔蛋白在体内对多糖特异性以及蛋白质特异性体液反应的有效免疫增强作用。

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