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首页> 外文期刊>Infection and immunity >Secretion of Shigella flexneri Ipa invasins on contact with epithelial cells and subsequent entry of the bacterium into cells are growth stage dependent.
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Secretion of Shigella flexneri Ipa invasins on contact with epithelial cells and subsequent entry of the bacterium into cells are growth stage dependent.

机译：弗氏志贺氏菌Ipa侵袭素在与上皮细胞接触后的分泌以及细菌随后进入细胞的过程取决于生长阶段。

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Upon contact with the surface of epithelial cells, Shigella flexneri secretes Ipa proteins through the Mxi-Spa type III secretion apparatus. Among the Ipa proteins, IpaB and IpaC form a soluble complex in the bacterial supernatant which appears to be sufficient to initiate the cellular rearrangements necessary to achieve bacterial entry. Here, we provide the first evidence that efficiency of bacterial entry into cells depends on the stage of bacterial growth. Bacteria in the early phase of exponential growth are six times more invasive than those in the stationary phase. The entry efficiency of the bacteria present on the cell surface appears to correlate with the percentage of those that are able to secrete their invasins. This suggests that the capacity to activate the Mxi-Spa apparatus is a major factor in the regulation of bacterial entry efficiency. Consistent with these observations, we have further shown that bacteria which have reached the stage of division secrete Ipa proteins more often than those that have not. Also, initial secretion occurs essentially in the area of the septation furrow. The Ipa proteins, secreted in the vicinity of the septation furrow, seem to initiate the early stages of reorganization of the host cell cytoskeleton.
机译：在与上皮细胞表面接触后，弗氏志贺氏菌通过Mxi-Spa III型分泌设备分泌Ipa蛋白。在Ipa蛋白中，IpaB和​​IpaC在细菌上清液中形成可溶性复合物，这似乎足以引发实现细菌进入所必需的细胞重排。在这里，我们提供了细菌进入细胞效率取决于细菌生长阶段的第一个证据。指数生长早期的细菌的侵袭性比固定时期的细菌高六倍。存在于细胞表面的细菌的进入效率似乎与能够分泌其入侵素的细菌的百分比相关。这表明激活Mxi-Spa仪器的能力是调节细菌进入效率的主要因素。与这些观察结果一致，我们进一步表明，达到分裂阶段的细菌比没有分裂的细菌分泌Ipa蛋白的频率更高。同样，最初的分泌基本上发生在分隔沟的区域。在分隔沟附近分泌的Ipa蛋白似乎启动了宿主细胞骨架的重组早期阶段。

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