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首页> 外文期刊>Infection and immunity >Resident Enteric Bacteria Are Necessary for Development of Spontaneous Colitis and Immune System Activation in Interleukin-10-Deficient Mice
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Resident Enteric Bacteria Are Necessary for Development of Spontaneous Colitis and Immune System Activation in Interleukin-10-Deficient Mice

机译：白细胞介素10缺乏症小鼠中自发性结肠炎和免疫系统活化的发展需要常驻肠道细菌。

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Mice with targeted deletion of the gene for interleukin-10 (IL-10) spontaneously develop enterocolitis when maintained in conventional conditions but develop only colitis when kept in specific-pathogen-free (SPF) environments. This study tested the hypothesis that enteric bacteria are necessary for the development of spontaneous colitis and immune system activation in IL-10-deficient mice. IL-10-deficient mice were maintained in either SPF conditions or germfree conditions or were populated with bacteria known to cause colitis in other rodent models. IL-10-deficient mice kept in SPF conditions developed colitis in all segments of the colon (cecum and proximal and distal colon). These mice exhibited immune system activation as evidenced by increased expression of CD44 on CD4+ T cells; increased mesenteric lymph node cell numbers; and increased production of immunoglobulin A (IgA), IgG1, and IL-12 p40 from colon fragment cultures. Mice populated with bacterial strains, including Bacteroides vulgatus, known to induce colitis in other rodent models had minimal colitis. Germfree IL-10-deficient mice had no evidence of colitis or immune system activation. We conclude therefore that resident enteric bacteria are necessary for the development of spontaneous colitis and immune system activation in IL-10-deficient mice.
机译：在常规条件下维持正常状态时，具有针对性白介素10（IL-10）基因缺失的小鼠会自发发展为小肠结肠炎，而在无特定病原体（SPF）的环境中则只会发展为结肠炎。这项研究检验了以下假设：在IL-10缺陷型小鼠中，肠细菌对于自发性结肠炎的发展和免疫系统的激活是必需的。 IL-10缺陷小鼠维持在SPF条件或无菌条件下，或在其他啮齿动物模型中填充已知导致结肠炎的细菌。保持SPF条件的IL-10缺陷小鼠在结肠的所有部分（盲肠以及近端和远端结肠）均发生结肠炎。这些小鼠表现出免疫系统激活，这可以通过CD4 + T细胞上CD44表达的增加来证明。肠系膜淋巴结细胞数量增加;结肠片段培养物中免疫球蛋白A（IgA），IgG1和IL-12 p40的产量增加。已知在其他啮齿动物模型中诱发细菌性结肠炎的细菌菌株包括 Bacteroides vulgatus 。 IL-10缺乏缺陷的小鼠没有结肠炎或免疫系统激活的证据。因此，我们得出结论，驻留肠细菌对于IL-10缺陷型小鼠的自发性结肠炎和免疫系统激活是必需的。

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