Heterogeneity in Levels of Vacuolating Cytotoxin Gene (vacA) Transcription among Helicobacter pylori Strains

M. H. Forsyth; J. C. Atherton; M. J. Blaser; T. L. Cover

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Heterogeneity in Levels of Vacuolating Cytotoxin Gene (vacA) Transcription among Helicobacter pylori Strains

机译：幽门螺杆菌菌株中空泡细胞毒素基因（vacA）转录水平的异质性。

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Broth culture supernatants from Tox+ Helicobacter pylori strains induce vacuolation of HeLa cells in vitro and contain VacA in concentrations that are higher than those found in supernatants from Tox? H. pyloristrains. To investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cytotoxin gene (vacA) in eight Tox+ strains (each with a type s1/m1 vacA genotype) and nine Tox? strains (each with a type s2/m2 vacA genotype). Most of the Tox+ and Tox? strains tested used the samevacA transcriptional start point, but Tox+strains yielded significantly stronger primer extension signal intensities than did Tox? strains (mean densitometry values of 15.8 ± 1.9 versus 8.9 ± 1.7, P = 0.0016). Correspondingly, when we introducedvacA::xylE transcriptional fusions into the chromosomes of a Tox+ strain (60190) and a Tox? strain (86-313), the level of XylE activity in 60190vacA::xylE was about 30-fold higher than that in 86-313 vacA::xylE. Sequence analysis and promoter exchange experiments indicated that the different levels of vacA transcription in these two strains cannot be explained solely by a difference in promoter strength. These data indicate that Tox+ and Tox? H. pylori strains typically differ not only in the VacA amino acid sequence but also in the level of vacA transcription.

机译：Tox + 幽门螺杆菌菌株的肉汤培养上清液在体外诱导HeLa细胞空泡化，其VacA的浓度高于Tox ？< / sup> H。幽门螺杆菌为了研究这种现象的基础，我们分析了八种Tox + 菌株（每个菌株均为s1 / m1 型）中空泡细胞毒素基因（ vacA ）的转录vacA 基因型）和9个Tox ？菌株（每个菌株均为s2 / m2 vacA 基因型）。测试的大多数Tox + 和Tox ？菌株都使用相同的 vacA 转录起点，但Tox + 菌株产生的引物延伸信号强度明显强于Tox ？菌株（平均光密度测定值为15.8±1.9对8.9±1.7， P = 0.0016）。相应地，当我们在Tox + 菌株（60190）和Tox 的染色体中引入 vacA :: xylE 转录融合体时？菌株（86-313），XylE活性在60190 vacA :: xylE 中比在86-313 vacA :: xylE 。序列分析和启动子交换实验表明，这两个菌株中 vacA 转录水平的不同不能仅仅通过启动子强度的差异来解释。这些数据表明Tox + 和Tox ？ H。幽门螺杆菌菌株不仅在VacA氨基酸序列上有所不同，而且在 vacA 转录水平上也有所不同。 展开▼

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《Infection and immunity》 |1998年第7期|共7页

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M. H. Forsyth; J. C. Atherton; M. J. Blaser; T. L. Cover;
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中图分类医学免疫学;

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专利

1. Vacuolating cytotoxin A (vacA) and cytotoxin-associated gene A (cagA) genotypes of resistant Helicobacter pylori strains isolated from raw and pasteurized milk [J] . Baradari Hamed, Mashak Zohreh, Tavakoli-Far Bahareh Journal of Food Safety . 2019,第1期

机译：真空的细胞毒素A（VACA）和细胞毒素相关基因A（CAGA）基因菌免受原始和巴氏乳牛奶分离的抗性幽门螺杆菌菌株的基因型

2. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. [J] . Z Xiang, S Censini, P F Bayeli, Infection and immunity . 1995,第1期

机译：分析43株幽门螺杆菌中CagA和VacA毒力因子的表达表明，临床分离株可分为两种主要类型，而CagA对于表达空泡细胞毒素不是必需的。

3. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. [J] . Z Xiang, S Censini, P F Bayeli, Infection and immunity . 1995,第1期

机译：分析43株幽门螺杆菌中CagA和VacA毒力因子的表达表明，临床分离株可分为两种主要类型，而CagA对于表达空泡细胞毒素不是必需的。

4. Study on cytotoxin associated gene of Helicobacter pylori and relationship to gastrointestinal diseases [C] . Wang Hongtao, Liu Jingxing, Wu Hong, 7th China-Japan International Congress of Microbiology Shanghai Symposium-2000 4-6 August 2000 Shanghai . 2000

机译：幽门螺杆菌细胞毒素相关基因及其与胃肠道疾病关系的研究

5. Structural, functional, and phylogenetic analyses of the Helicobacter pylori vacuolating toxin (VacA). [D] . Gangwer. Kelly A. 2010

机译：幽门螺杆菌空泡毒素（VacA）的结构，功能和系统发育分析。

6. Heterogeneity in Levels of Vacuolating Cytotoxin Gene (vacA) Transcription among Helicobacter pylori Strains [O] . M. H. Forsyth, J. C. Atherton, M. J. Blaser, 1998

机译：幽门螺杆菌菌株中空泡细胞毒素基因（vacA）转录水平的异质性。

7. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin [O] . Z Xiang, S Censini, P F Bayeli, 1995

机译：Caga和Vaca毒力因子表达分析43株幽门螺杆菌揭示临床分离株可以分为两种主要类型，并且Caga对真空细胞毒素的表达不是必需的

1. 幽门螺杆菌空泡细胞毒素(Vacuolating cytotoxin,VacA)研究进展 [J] . 赵雩卿 ,钱家鸣 . 胃肠病学和肝病学杂志 . 2004,第003期

2. 幽门螺杆菌细胞毒素相关蛋白A、细胞毒素相关蛋白E、细胞空泡毒素A基因型与上消化道疾病的关系 [J] . 蒋闵仁 ,黄英 ,宋佳鸿 . 实用临床医药杂志 . 2017,第013期

3. 台湾病人的幽门螺杆菌细胞毒素相关基因和空泡毒素基因的基因分析 [J] . Yang.JC ,Wang.TH . 世界医学杂志 . 1998,第008期

4. 幽门螺杆菌细胞毒素相关基因A、空泡毒素基因A抗体与胃十二指肠疾病的关系 [J] . 张丽萍 ,张岫兰 ,卢启明 . 兰州大学学报（医学版） . 2008,第002期

5. 幽门螺杆菌细胞毒素相关基因、空泡毒素基因及其表达产物与儿童胃十二指肠疾病的关系 [J] . 陈肖肖 ,尚世强 ,欧弼悠 . 中华实用儿科临床杂志 . 2003,第011期

6. 幽门螺杆菌产毒菌株CagA、VacA与慢性胃病关系的研究 [C] . 王冠庭 . 第十三次全国消化系统疾病学术研讨会 . 2001

7. 浙江地区幽门螺杆菌临床菌株cagA-vacA优势基因型及不同基因型菌株混合感染的调查 [A] . 陈学军 . 2003

1. 一种检测幽门螺杆菌空泡毒素vacA的LAMP引物组及其应用 [P] . 中国专利： CN108728517A . 2018-11-02

2. 幽门螺杆菌vacA－B基因表达工程菌的构建及其产物的制备方法 [P] . 中国专利： CN1358862A . 2002-07-17

3. A POOL OF RECOMBINANT HELICOBACTER PYLORI VACUOLATING CYTOTOXIN FRAGMENTS FROM E.COLI FOR VACCINE [P] . 外国专利： KR20080009998A . 2008-01-30

机译：一株重组幽门螺杆菌从大肠杆菌中提取细胞毒素片段的疫苗

4. A pool of recombinant Helicobacter pylori vacuolating cytotoxin fragments from E.coli for vaccine [P] . 外国专利： KR100845043B1 . 2008-07-08

机译：重组幽门螺杆菌空泡大肠杆菌中的细胞毒素片段用于疫苗

5. dapE gene on Helicobacter pylori and dapE mutant strains of Helicobacter pylori [P] . 外国专利： US6570004B1 . 2003-05-27

机译：幽门螺杆菌的dapE基因和幽门螺杆菌的dapE突变株

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Heterogeneity in Levels of Vacuolating Cytotoxin Gene (vacA) Transcription among Helicobacter pylori Strains

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