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首页> 外文期刊>Infection and immunity >DNA and a CpG Oligonucleotide Derived fromBabesia bovis Are Mitogenic for Bovine B Cells
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DNA and a CpG Oligonucleotide Derived fromBabesia bovis Are Mitogenic for Bovine B Cells

机译：牛肝杆菌衍生的DNA和CpG寡核苷酸对牛B细胞有丝分裂作用。

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DNAs from bacteria and variety of nonvertebrate organisms, including nematodes, mollusks, yeasts, and insects, cause polyclonal activation of murine B lymphocytes. Similar studies have not been reported for bovine B cells, and to date no studies have reported mitogenic properties of protozoal DNA for any species. However, we and others have observed that protozoal parasite antigens can induce the proliferation of lymphocytes from nonexposed donors. Extending these studies, we now show that the mitogenic property of protozoal antigen preparations is in part attributable to parasite DNA and thatBabesia bovis DNA is directly mitogenic for bovine B cells. DNase treatment of B. bovis extracts abrogated B. bovis-induced proliferation of peripheral blood mononuclear cells from nonexposed cattle. Like DNAs from other organisms that were mitogenic for murine B cells, B. bovis DNA is largely nonmethylated and induced a dose-dependent proliferation of bovine B cells, which was reduced upon methylation. Furthermore, B. bovis and E. coli DNAs enhanced immunoglobulin secretion by cultured B cells, inducing moderate increases in immunoglobulin G1 and stronger increases in immunoglobulin G2. Because certain nonmethylated CpG motifs present in bacterial DNA are known to stimulate proliferation of murine and human B cells, an 11-kb fragment of B. bovis DNA was analyzed for CG dinucleotide content and for the presence of known immunostimulatory sequences (ISS) centered on a CG motif. The frequency of CG dinucleotides was approximately one-half of the expected frequency, and several CpG hexameric sequences with known activity for murine B cells were identified. An oligodeoxynucleotide containing one of these ISS (AACGTT), which is present within the rhoptry-associated protein-1 (rap-1) open reading frame, was shown to stimulate B-cell proliferation. These ISS may be involved in host immune modulation during protozoal infection and may be useful as vaccine adjuvants.
机译：来自细菌和各种非脊椎动物的DNA（包括线虫，软体动物，酵母和昆虫）的DNA导致鼠B淋巴细胞的多克隆激活。尚未针对牛B细胞进行过类似的研究，迄今为止，还没有任何研究报道过原生动物DNA对任何物种的促有丝分裂特性。但是，我们和其他人已经观察到，原生动物的寄生虫抗原可以诱导未暴露供体的淋巴细胞增殖。扩展这些研究，我们现在表明，原生动物抗原制剂的促有丝分裂特性部分归因于寄生虫DNA，而 Bobessia bovis DNA对牛B细胞直接促有丝分裂。 DNase处理 B。 bovis 提取废除的 B。牛诱导的未暴露牛外周血单个核细胞增殖。就像来自其他生物的DNA一样，它们对鼠B细胞有丝分裂作用。牛DNA大部分未甲基化，并诱导了牛B细胞的剂量依赖性增殖，甲基化后这种活性降低。此外， B。 bovis 和 E。大肠杆菌DNA增强了培养的B细胞的免疫球蛋白分泌，诱导了免疫球蛋白G1的适度增加，并增强了免疫球蛋白G2的增加。因为已知细菌DNA中存在某些未甲基化的CpG基序可以刺激鼠和人B细胞的增殖，所以11kb的B基因片段。分析牛牛的DNA中CG二核苷酸的含量，以及是否存在以CG基序为中心的已知免疫刺激序列（ISS）。 CG二核苷酸的频率约为预期频率的一半，并且鉴定出了几种对鼠B细胞具有已知活性的CpG六聚体序列。含有这些ISS之一的寡聚脱氧核苷酸（AACGTT）存在于rhoptry相关蛋白1（ rap-1 ）开放阅读框中，被证明能刺激B细胞增殖。这些ISS可能参与原生动物感染期间的宿主免疫调节，并可用作疫苗佐剂。

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