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首页> 外文期刊>Infection and immunity >Complete DNA Sequence and Detailed Analysis of the Yersinia pestis KIM5 Plasmid Encoding Murine Toxin and Capsular Antigen
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Complete DNA Sequence and Detailed Analysis of the Yersinia pestis KIM5 Plasmid Encoding Murine Toxin and Capsular Antigen

机译：鼠疫耶尔森氏菌编码鼠毒素和荚膜抗原的鼠疫KIM5质粒的完整DNA序列和详细分析

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Yersinia pestis, the causative agent of plague, harbors at least three plasmids necessary for full virulence of the organism, two of which are species specific. One of the Y. pestis-specific plasmids, pMT1, is thought to promote deep tissue invasion, resulting in more acute onset of symptoms and death. We determined the entire nucleotide sequence of Y. pestis KIM5 pMT1 and identified potential open reading frames (ORFs) encoded by the 100,990-bp molecule. Based on codon usage for known yersinial genes, homology with known proteins in the databases, and potential ribosome binding sites, we determined that 115 of the potential ORFs which we considered could encode polypeptides in Y. pestis. Five of these ORFs were genes previously identified as being necessary for production of the classic virulence factors, murine toxin (MT), and the fraction 1 (F1) capsule antigen. The regions of pMT1 encoding MT and F1 were surrounded by remnants of multiple transposition events and bacteriophage, respectively, suggesting horizontal gene transfer of these virulence factors. We identified seven new potential virulence factors that might interact with the mammalian host or flea vector. Forty-three of the remaining 115 putative ORFs did not display any significant homology with proteins in the current databases. Furthermore, DNA sequence analysis allowed the determination of the putative replication and partitioning regions of pMT1. We identified a single 2,450-bp region within pMT1 that could function as the origin of replication, including a RepA-like protein similar to RepFIB, RepHI1B, and P1 and P7 replicons. Plasmid partitioning function was located ca. 36 kb from the putative origin of replication and was most similar to the parABS bacteriophage P1 and P7 system. Y. pestis pMT1 encoded potential genes with a high degree of similarity to a wide variety of organisms, plasmids, and bacteriophage. Accordingly, our analysis of the pMT1 DNA sequence emphasized the mosaic nature of this large bacterial virulence plasmid and provided implications as to its evolution.
机译：鼠疫的致病性鼠疫耶尔森菌含有至少三种对生物完全毒力所必需的质粒，其中两个是物种特异性的。 Y之一。鼠疫菌特异性质粒pMT1被认为可促进深部组织浸润，从而导致更严重的症状发作和死亡。我们确定了 Y的完整核苷酸序列。鼠疫 KIM5 pMT1并鉴定出由100,990-bp分子编码的潜在开放阅读框（ORF）。基于已知的耶尔森基因的密码子使用，与数据库中已知蛋白质的同源性以及潜在的核糖体结合位点，我们确定了我们认为的115个潜在ORF可编码 Y中的多肽。瘟疫。这些ORF中有五个是先前鉴定为产生经典毒力因子，鼠毒素（MT）和级分1（F1）胶囊抗原所必需的基因。编码MT和F1的pMT1区域分别被多个转座事件和噬菌体的残余物包围，表明这些毒力因子的水平基因转移。我们确定了可能与哺乳动物宿主或跳蚤载体相互作用的七个新的潜在毒力因子。其余115个推定的ORF中有43个与当前数据库中的蛋白质没有显示出任何显着同源性。此外，DNA序列分析允许确定pMT1的假定复制和分配区域。我们在pMT1中鉴定了一个单个的2,450 bp区域，它可以作为复制起点，包括类似于RepFIB，RepHI1B以及P1和P7复制子的RepA样蛋白。质粒分配功能位于约。从假定的复制起点起36 kb，与 parABS 噬菌体P1和P7系统最为相似。是的鼠疫pMT1编码的潜在基因与多种生物，质粒和噬菌体高度相似。因此，我们对pMT1 DNA序列的分析强调了这种大细菌毒力质粒的镶嵌性质，并对其进化提供了启示。

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