Inhibition of binding, entry, or intracellular proliferation of Ehrlichia risticii in P388D1 cells by anti-E. risticii serum, immunoglobulin G, or Fab fragment.

J B Messick; Y Rikihisa

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Inhibition of binding, entry, or intracellular proliferation of Ehrlichia risticii in P388D1 cells by anti-E. risticii serum, immunoglobulin G, or Fab fragment.

机译：抗E抑制P388D1细胞中的埃希氏毛虫结合，进入或细胞内增殖。 Risticii血清，免疫球蛋白G或Fab片段。

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The effects of equine antiserum, immunoglobulin G (IgG) specific for Ehrlichia risticii, and its Fab fragment on E. risticii binding to, internalization into, and proliferation in P388D1 cells were studied by immunofluorescence flow cytometry. Anti-E. risticii equine serum or IgG inhibited E. risticii at a stage beyond binding and internalization. In contrast, monovalent anti-E. risticii equine Fab fragments inhibited E. risticii binding and internalization into P388D1 cells. In the presence of control equine serum, IgG, or its Fab fragment, E. risticii cells were bound, were internalized and subsequently grew within P388D1 cells, and eventually destroyed the host cells as effectively as was the case without equine serum, IgG, or Fab fragments. Anti-E. risticii IgG but not normal horse IgG inhibited L-[14C]glutamine metabolism in Percoll gradient-purified E. risticii. These findings suggest that the Fab fragment of intact anti-E. risticii IgG blocks the ligands on E. risticii responsible for non-IgG-mediated internalization and diverts them to bind via the Fc receptor. Following Fc-mediated entry of E. risticii, the antibody interfered with the metabolic activity of E. risticii cells, rendering them incapable of proliferation in P388D1 cells and resulting in the eventual destruction of the organisms.

机译：通过免疫荧光流式细胞术研究了马抗血清，对埃希氏毛虫的特异性免疫球蛋白G（IgG）及其Fab片段对埃希氏毛虫结合，内化和在P388D1细胞中增殖的影响。反E。 Risticii马血清或IgG在超出结合和内在化的阶段抑制了E. risticii。相反，单价抗E。栗色马的Fab片段抑制了栗色大肠杆菌的结合并内化到P388D1细胞中。在存在对照马血清，IgG或其Fab片段的情况下，结合大肠杆菌，将其内化并随后在P388D1细胞内生长，并最终像不使用马血清，IgG或E一样有效地破坏宿主细胞。 Fab片段。反E。 risticii IgG，但正常马IgG不抑制Percoll梯度纯化的E.risticii中的L- [14C]谷氨酰胺代谢。这些发现表明完整的抗E的Fab片段。 risticii IgG阻断了E.risticii上的配体，这些配体负责非IgG介导的内化作用，并转移它们通过Fc受体结合。 Fc介导的埃里希氏菌进入后，抗体干扰埃里希氏菌细胞的代谢活性，使其无法在P388D1细胞中增殖，并最终破坏了生物。

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《Infection and immunity》 |1994年第8期|共6页
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J B Messick; Y Rikihisa;
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中图分类医学免疫学;
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1. Lack of lysosomal fusion with phagosomes containing Ehrlichia risticii in P388D1 cells: abrogation of inhibition with oxytetracycline. [J] . M Y Wells, Y Rikihisa Infection and immunity . 1988,第12期

机译：P388D1细胞缺乏与溶血性埃里希氏体吞噬体的溶酶体融合：用土霉素消除抑制作用。
2. Presence of parasite antigen on the surface of P388D1 cells infected with Ehrlichia risticii. [J] . J B Messick, Y Rikihisa Infection and immunity . 1992,第8期

机译：在感染埃希氏毛虫的P388D1细胞表面上存在寄生虫抗原。
3. Characterization of Ehrlichia risticii binding, internalization, and proliferation in host cells by flow cytometry. [J] . J B Messick, Y Rikihisa Infection and immunity . 1993,第9期

机译：流式细胞仪表征寄主埃希氏菌在宿主细胞中的结合，内在化和增殖。
4. PROLIFERATION OF ANTIGEN BINDING CELLS AND IMMUNOGLOBULIN BEARING CELLS AND CASE STUDIES IN THE USE OF SCIENTIFIC INFORMATION IN SOCIAL DECISION-MAKING: LEAD-ARSENATE AS AN URBAN INSECTICIDE AND SODIUM-NITRITE AS A FOOD ADDITIVE [D] . HATTIS, DALE BEN. -1

机译：抗原结合细胞和免疫球蛋白的增殖及在社会决策中使用科学信息的案例研究：砷作为城市杀虫剂，亚硝酸钠作为食品添加剂
5. Inhibition of binding entry or intracellular proliferation of Ehrlichia risticii in P388D1 cells by anti-E. risticii serum immunoglobulin G or Fab fragment. [O] . J B Messick, Y Rikihisa 1994

机译：抗E抑制P388D1细胞中的埃希氏毛虫结合进入或细胞内增殖。 risticii血清免疫球蛋白G或Fab片段。
6. Lack of lysosomal fusion with phagosomes containing Ehrlichia risticii in P388D1 cells: abrogation of inhibition with oxytetracycline [O] . M Y Wells, Y Rikihisa 1988

机译：在P388D1细胞中缺乏含有EHRLICHIA RISTICII的杀虫剂的溶酶体融合：用催产素消除抑制作用

Inhibition of binding, entry, or intracellular proliferation of Ehrlichia risticii in P388D1 cells by anti-E. risticii serum, immunoglobulin G, or Fab fragment.

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