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首页> 外文期刊>Infection and immunity >Cytopathic effects of Treponema denticola chymotrypsin-like proteinase on migrating and stratified epithelial cells.
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Cytopathic effects of Treponema denticola chymotrypsin-like proteinase on migrating and stratified epithelial cells.

机译：密螺旋体胰凝乳蛋白酶样蛋白酶对迁移和分层的上皮细胞的细胞病变作用。

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The effects of Treponema denticola and its outer membrane-bound chymotrypsin-like proteinase on periodontal ligament epithelial cell cultures at different stages of maturity were studied. In sparse cultures with migrating epithelial cells, large intracellular vacuoles were formed rapidly following exposure to live T. denticola. Treponemes showing structural damage were seen occasionally inside membrane-bound vesicles. Intensive membrane blebbing occurred in infected cells and continued for up to 48 h before the cell died. Blebbing could also be induced by a purified chymotrypsin-like proteinase of T. denticola. Cortical actin and alpha-actinin of the bacterium-treated cells showed disorganization, and pericellular fibronectin was degraded by both whole T. denticola and the isolated proteinase. Epithelial cells with well-formed lateral cell contacts appeared to be more resistant to the effects of T. denticola than migrating isolated cells. In multilayer epithelial cultures, adhesion of T. denticola and membrane blebbing were observed infrequently. There was no evidence of invasion of T. denticola into epithelial multilayers. However, immunogold electron microscopy showed rapid transport of T. denticola chymotrypsin-like proteinase into newly formed large intracellular vacuoles within the epithelial layers. These vacuoles were lined by membranes studded with ribosomes. T. denticola-treated epithelial multilayers had loose cell contacts, collapsed intercellular spaces, and increased permeability. Through its capacity to cause these unique cytopathic effects, the chymotrypsin-like proteinase of T. denticola has the potential to contribute to the initiation of periodontal disease.
机译：研究了密螺旋体及其外膜结合型胰凝乳蛋白酶样蛋白酶对不同成熟期牙周膜上皮细胞培养的影响。在具有迁移的上皮细胞的稀疏培养物中，暴露于活的T. denticola后迅速形成大的细胞内液泡。偶尔在膜结合的囊泡中发现显示结构损伤的线粒体。在感染的细胞中发生强烈的膜起泡，并持续长达48小时，然后细胞死亡。提提克氏菌的纯化的胰凝乳蛋白酶样蛋白酶也可能引起出血。细菌处理过的细胞的皮质肌动蛋白和α-肌动蛋白显示出组织紊乱，并且整个树状体和分离的蛋白酶均降解了细胞周围的纤连蛋白。与迁移分离的细胞相比，具有良好的横向细胞接触的上皮细胞似乎对树状体的抗药性更高。在多层上皮培养物中，很少观察到T. denticola的粘附和膜起泡。没有证据表明T. denticola侵入上皮多层细胞。然而，免疫金电子显微镜检查显示，树突触菌凝乳胰蛋白酶样蛋白酶迅速转移到上皮层内新形成的大细胞液泡中。这些液泡衬有布满核糖体的膜。 T. denticola处理的上皮多层细胞接触松散，细胞间隙塌陷，通透性增加。通过其引起这些独特的细胞病变作用的能力，树突触菌的胰凝乳蛋白酶样蛋白酶具有促进牙周疾病发作的潜力。

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