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首页> 外文期刊>Infection and immunity >Binding of Haemophilus influenzae to purified mucins from the human respiratory tract.
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Binding of Haemophilus influenzae to purified mucins from the human respiratory tract.

机译：流感嗜血杆菌与人类呼吸道纯化的黏蛋白的结合。

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Mucins are high-molecular-weight glycoproteins and major constituents of the mucus layer which covers the airway surface. We have studied the interactions between bacteria, mucins, and epithelial cells from the human respiratory tract. Nontypeable strains of Haemophilus influenzae were found to bind to purified airway mucins in suspension and on solid phase. Mucins in suspension inhibited the attachment of these strains to nasopharyngeal epithelial cells, while mucin coating of the cells enhanced their binding. In contrast, strains of Streptococcus pneumoniae and encapsulated and other nontypeable H. influenzae strains failed to interact with mucins. These H. influenzae strains used other strategies for adherence to epithelial cells. The type b strain 770235 attached via fimbriae but also expressed a subcapsular adhesin that was detected in a capsule- and fimbria-defective mutant. Mucin pretreatment of these bacteria did not inhibit adherence, but mucin pretreatment of epithelial cells inhibited adherence, probably by shielding of the receptors for these adhesins. Non-mucin-binding nontypeable and encapsulated H. influenzae strains would, therefore, adhere only after disruption of the mucus layer and exposure of cellular receptors. Differences in tissue toxicity and invasiveness among H. influenzae strains may also be influenced by the mucin interactions of the strains.
机译：粘蛋白是高分子量的糖蛋白，是覆盖气道表面的粘液层的主要成分。我们已经研究了细菌，粘蛋白和人类呼吸道上皮细胞之间的相互作用。发现流感嗜血杆菌的不可分型菌株在悬浮液中和在固相上与纯化的气道粘蛋白结合。悬浮液中的粘蛋白抑制了这些菌株对鼻咽上皮细胞的附着，而细胞的粘蛋白涂层增强了它们的结合。相比之下，肺炎链球菌菌株和封装的以及其他不可分型的流感嗜血杆菌菌株均无法与粘蛋白相互作用。这些流感嗜血杆菌菌株使用其他策略粘附于上皮细胞。 b型菌株770235通过菌毛附着，但也表达了在荚膜和菌毛缺陷的突变体中检测到的荚膜下粘附素。这些细菌的粘蛋白预处理不能抑制粘附，但是上皮细胞的粘蛋白预处理可以抑制粘附，可能是通过屏蔽这些粘附素的受体来实现的。因此，非粘蛋白结合的不可分型和封装的流感嗜血杆菌菌株仅在破坏粘液层和暴露细胞受体后才粘附。流感嗜血杆菌菌株之间组织毒性和侵袭性的差异也可能受到菌株粘蛋白相互作用的影响。

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