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首页> 外文期刊>Infection and immunity >Immunogenicity of synthetic peptides of Haemophilus influenzae type b outer membrane protein P1.
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Immunogenicity of synthetic peptides of Haemophilus influenzae type b outer membrane protein P1.

机译：b型流感嗜血杆菌外膜蛋白P1合成肽的免疫原性。

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To identify the B- and T-cell epitopes of P1 of Haemophilus influenzae type b, 13 peptides covering 90% of the protein were chemically synthesized. Mouse, guinea pig, and rabbit antisera raised against purified native P1 were tested for their reactivities against the peptides in peptide-specific enzyme-linked immunosorbent assays (ELISAs). Six immunodominant linear B-cell epitopes were mapped to residues 103 to 137, 189 to 218, 248 to 283, 307 to 331, 384 to 412, and 400 to 437 of the mature P1 protein. When P1 peptides were screened for their reactivities with three human convalescent-phase serum specimens, peptides corresponding to residues 39 to 64, 226 to 253, and 400 to 437 reacted strongly with the antisera. Four regions (residues 39 to 64, 226 to 253, 339 to 370, and 400 to 437) contained murine T-cell epitopes. Rabbit antipeptide antisera were tested for their reactivities with the immunizing peptides and P1 protein by ELISA and immunoblots. All anti-P1 peptide antisera except those raised against peptide HIBP1-8 (residues 279 to 312) or HIBP1-8-keyhole limpet hemocyanin conjugate were shown to be specific for their respective immunizing peptides by ELISA. In addition, rabbit antisera raised against the synthetic peptides corresponding to residues 1 to 29, 39 to 64, 103 to 137, 189 to 218, 226 to 253, 248 to 283, 307 to 331, and 400 to 437 of the mature P1 protein recognized the P1 protein from both typeable and nontypeable isolates. These results suggest that these peptides contain epitopes highly conserved among typeable and nontypeable strains of H. influenzae. However, none of the antipeptide antisera have bactericidal activity, nor were they protective against H. influenzae type b in the infant rat model of bacteremia.
机译：为了鉴定b型流感嗜血杆菌的B细胞和T细胞表位，化学合成了覆盖90％蛋白质的13种肽。在肽特异性酶联免疫吸附试验（ELISA）中测试了针对纯化的天然P1产生的小鼠，豚鼠和兔抗血清对肽的反应性。将六个免疫优势线性B细胞表位定位到成熟P1蛋白的残基103至137、189至218、248至283、307至331、384至412和400至437。当用三个人类恢复期血清样品筛选P1肽的反应性时，对应于残基39至64、226至253和400至437的肽与抗血清强烈反应。四个区域（残基39至64、226至253、339至370和400至437）包含鼠T细胞表位。通过ELISA和免疫印迹测试了兔抗肽抗血清与免疫肽和P1蛋白的反应性。通过ELISA显示，除针对肽HIBP1-8（残基279至312）或HIBP1-8-匙孔戚血蓝蛋白缀合物产生的抗P1肽抗血清外，它们均对各自的免疫肽具有特异性。另外，兔抗血清针对对应于成熟P1蛋白的残基1至29、39至64、103至137、189至218、226至253、248至283、307至331和400至437的合成肽产生从可分型和不可分型的分离物中识别出P1蛋白。这些结果表明，这些肽含有在流感嗜血杆菌的可分型和不可分型菌株中高度保守的表位。但是，在婴儿菌血症大鼠模型中，抗肽抗血清都没有杀菌活性，也没有针对b型流感嗜血杆菌的保护作用。

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