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首页> 外文期刊>Infection and immunity >Cytokine production by human epithelial and endothelial cells following exposure to oral viridans streptococci involves lectin interactions between bacteria and cell surface receptors.
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Cytokine production by human epithelial and endothelial cells following exposure to oral viridans streptococci involves lectin interactions between bacteria and cell surface receptors.

机译：人口上皮细胞和内皮细胞暴露于口服绿脓杆菌链球菌后产生细胞因子涉及细菌与细胞表面受体之间的凝集素相互作用。

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In order to examine the possible implication of human epithelial and endothelial cells in the pathogenesis of various diseases associated with oral viridans streptococci, we tested the immunomodulatory effects of 11 representative strains of oral viridans streptococci on human epithelial KB cells and endothelial cells. We then examined the possible role of two major adhesins from oral viridans streptococci, protein I/II and rhamnose-glucose polymers (RGPs), in this process. In this study we demonstrate that oral viridans streptococci are potent stimulators of interleukin-8 (IL-8) production from KB cells and of IL-6 and IL-8 production from endothelial cells. The ability of protein I/II and RGPs to contribute to these effects was then examined. Using biotinylated protein I/IIf and RGPs from Streptococcus mutans OMZ 175, we showed that these adhesins bind to KB and endothelial cells through specific interactions and that the binding of these molecules initiates the release of IL-8 from KB cells and of IL-6 and IL-8 from endothelial cells. These results suggest that protein I/IIf and RGPs play an important role in the interactions between bacteria and KB and endothelial cells in that similar cytokine profiles are obtained when cells are stimulated with bacteria or surface components. We also provide evidence that protein I/IIf binds to and stimulates KB and endothelial cells through lectin interactions and that N-acetyl neuraminic acid (NANA) and fucose present on cell surface glycoproteins may form the recognition site since binding and cytokine release can be inhibited by dispase and periodate treatment of cells and by NANA and fucose. These results demonstrate that oral viridans streptococci, probably by engaging two cell surface adhesins, exert immunomodulatory effects on human KB and endothelial cells.
机译：为了检查人类上皮细胞和内皮细胞在与口腔弧菌链球菌有关的各种疾病的发病机制中的可能意义，我们测试了11种代表性口腔弧菌链球菌菌株对人上皮KB细胞和内皮细胞的免疫调节作用。然后，我们研究了口腔粘膜链球菌中两种主要粘附素，蛋白质I / II和鼠李糖-葡萄糖聚合物（RGP）在此过程中的可能作用。在这项研究中，我们证明口服绿巨人链球菌是从KB细胞产生白介素8（IL-8）以及从内皮细胞产生IL-6和IL-8的有效刺激剂。然后检查了蛋白质I / II和RGP促成这些作用的能力。使用来自变形链球菌OMZ 175的生物素化蛋白I / IIf和RGP，我们显示这些黏附素通过特异性相互作用与KB和内皮细胞结合，并且这些分子的结合启动了IL-8从KB细胞和IL-6的释放和IL-8来自内皮细胞。这些结果表明，蛋白质I / IIf和RGP在细菌与KB和内皮细胞之间的相互作用中起着重要作用，因为当用细菌或表面成分刺激细胞时，可获得相似的细胞因子谱。我们还提供证据表明蛋白I / IIf通过凝集素相互作用结合并刺激KB和内皮细胞，并且存在于细胞表面糖蛋白上的N-乙酰神经氨酸（NANA）和岩藻糖可能形成识别位点，因为结合和细胞因子的释放可以被抑制通过分散和高碘酸盐处理细胞，以及NANA和岩藻糖。这些结果表明，口服翠绿链球菌可能通过使两种细胞表面粘附素结合而对人KB和内皮细胞产生免疫调节作用。

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