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首页> 外文期刊>Infection and immunity >Human immunoglobulin E responses to a recombinant 22.6-kilodalton antigen from Schistosoma mansoni adult worms are associated with low intensities of reinfection after treatment.
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Human immunoglobulin E responses to a recombinant 22.6-kilodalton antigen from Schistosoma mansoni adult worms are associated with low intensities of reinfection after treatment.

机译：人类免疫球蛋白E对曼氏血吸虫成虫的重组22.6千达尔顿抗原的反应与治疗后的低强度感染有关。

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摘要

Schistosoma mansoni-infected individuals who have low intensities of reinfection following treatment produce immunoglobulin E (IgE) antibodies against a range of S. mansoni adult-worm antigens. One of the targets of the IgE response is an adult-worm sodium dodecyl sulfate-polyacrylamide gel electrophoresis band of 22 kDa (Sm22), which contains an antigen(s) located within the tegument and gut lining of adult worms and relatively late schistosomula life cycle stages only. A significant negative correlation between the level of anti-Sm22 IgE and the intensity of reinfection following treatment suggests that IgE responses against this antigen(s) are characteristic of individuals who are resistant to reinfection. To identify the antigen(s) in the Sm22 band that are associated with these IgE responses, we have cloned and characterized a recombinant 22-kDa protein (rSm22) that cross-reacts immunologically with Sm22. There was a high correlation between native and recombinant Sm22 isotype responses, indicating that the correct antigen had been cloned and that responses against rSm22 made up the majority of the responses against Sm22. By analyzing human isotype responses to rSm22 with human sera from a longitudinal treatment and reinfection study and correlating the anti-rSm22 isotype responses, retrospectively, with the intensity of reinfection following treatment for each individual, we observed a negative correlation between the IgE response to rSm22 and the intensity of reinfection. This relationship remained significant after allowing for age and other isotype responses to rSm22, in particular IgG4.
机译：在治疗后再感染强度低的曼氏血吸虫感染的个体会产生针对多种曼氏梭菌成虫蠕虫抗原的免疫球蛋白E（IgE）抗体。 IgE应答的目标之一是22kDa（Sm22）的成虫蠕虫十二烷基硫酸钠-聚丙烯酰胺凝胶电泳带，其中包含位于成虫蠕虫和肠壁内的抗原，并且血吸虫的寿命相对较晚仅循环阶段。抗Sm22 IgE的水平与治疗后再感染强度之间的显着负相关表明，针对此抗原的IgE反应是抵抗再感染的个体的特征。为了鉴定与这些IgE反应相关的Sm22带中的抗原，我们已经克隆并鉴定了与Sm22发生免疫反应的重组22-kDa蛋白（rSm22）。天然和重组Sm22同种型应答之间存在高度相关性，表明已克隆了正确的抗原，并且针对rSm22的应答占了针对Sm22的应答的大部分。通过对来自纵向治疗和再感染研究的人血清对人rSm22的同种型反应进行分析，并将抗rSm22同种型的反应与每个个体治疗后的再感染强度相关联，我们观察到IgE对rSm22的反应之间呈负相关和再感染的强度。在考虑到年龄和其他对rSm22，特别是IgG4的同种型反应后，这种关系仍然很重要。

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