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首页> 外文期刊>Infection and immunity >Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient individuals.
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Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient individuals.

机译：大肠杆菌在缺乏免疫球蛋白A的人的结肠菌群中表达的甘露糖特异性粘附素减少。

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Most Escherichia coli isolates can express type 1 fimbriae with mannose-specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type 1 fimbriae. The absence of secretory IgA in colonic secretions, as seen in IgA deficiency, might therefore alter the ability of type 1-fimbriated E. coli to colonize the large intestines of these individuals. In the present study, 10 E. coli isolates from each of 17 IgA-deficient and 17 age-matched control individuals were assessed for the carriage of the fim gene cluster by DNA-DNA hybridization and for the expression of type 1 fimbriae by hemagglutination of guinea pig erythrocytes. The contribution of type 1-fimbria-mediated adherence to HT-29 colonic cells was also analyzed. The proportion of fim+ E. coli isolates was lower in IgA-deficient than in control individuals (74 versus 94%, P < 0.05), as was the proportion of isolates expressing type 1 fimbriae in vitro (69% versus 85%, P < 0.05). The median mannose-sensitive adherence to HT-29 cells was lower for isolates from IgA-deficient individuals than from the controls (9 versus 26 bacteria per cell, P < 0.05). Isolates expressing type 1 fimbriae showed lower adherence to HT-29 cells when they were derived from IgA-deficient individuals than when they were derived from control individuals (15 versus 27 bacteria per cell, P < 0.05). The results suggest that the interaction of type 1 fimbriae with secretory IgA contributes to the large intestinal colonization by these bacteria.
机译：大多数大肠杆菌分离株可以表达具有甘露糖特异性粘附素的1型菌毛。这些粘附素与分泌型免疫球蛋白A（IgA）的寡糖链结合。因此，除了特异性的抗体活性，分泌型IgA还具有与表达1型菌毛的细菌的广泛反应性。如在IgA缺乏症中所见，结肠分泌物中不存在分泌型IgA，因此可能会改变1型成纤维的大肠杆菌在这些个体的大肠中定殖的能力。在本研究中，通过DNA-DNA杂交评估了17个IgA缺陷和17个年龄匹配的对照个体中每个菌株的10个大肠杆菌分离物对fim基因簇的运输和对1型菌毛的血凝反应。豚鼠红细胞。还分析了1型纤维介导的粘附对HT-29结肠细胞的贡献。缺乏IgA的fim +大肠杆菌分离株的比例低于对照组（74比94％，P <0.05），在体外表达1型菌毛的分离株的比例也较低（69％比85％，P <0.05）。 0.05）。对于来自IgA缺陷型个体的分离株，其对HT-29细胞的中值甘露糖敏感性依从性低于对照（每细胞9个细菌与26个细菌，P <0.05）。表达1型菌毛的分离株对HT-29细胞的粘附力比对IgA缺陷个体的粘附力低（与15个细菌对27个细菌相比，P <0.05）。结果表明1型菌毛与分泌型IgA的相互作用有助于这些细菌在大肠中定植。

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