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首页> 外文期刊>Infection and immunity >Subcellular localization and functional characterization of Nc-p43, a major Neospora caninum tachyzoite surface protein.
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Subcellular localization and functional characterization of Nc-p43, a major Neospora caninum tachyzoite surface protein.

机译：Nc-p43（一种主要的新孢子虫速殖子表面蛋白）的亚细胞定位和功能表征。

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摘要

Neospora caninum is a recently identified coccidian parasite which shares many features with, but is clearly distinct from, Toxoplasma gondii. N. caninum tachyzoites infect a wide range of mammalian cells both in vivo and in vitro. The mechanisms by which infection is achieved are largely unknown. Recent evidence has suggested that a receptor-ligand system in which one or several host cell receptors bind to one or several parasite ligands is involved. Parasite cell surface-associated molecules such as the recently identified Nc-p43 antigen are prime suspects for being implicated in this physical interaction. In this study it is shown that invasion of Vero cell monolayers by N. caninum tachyzoites in vitro is impaired on incubation of parasites with subagglutinating amounts of affinity-purified antibodies directed against Nc-p43. Postembedding immunogold labeling with anti-Nc-p43 antibodies demonstrated that Nc-p43 is localized not only on the parasite cell surface but also within dense granules and rhoptries. The fate of Nc-p43 during intracellular proliferation of N. caninum tachyzoites and subsequent maturation of the parasitophorous vacuole was also studied.
机译：犬新孢子虫是最近鉴定的球虫寄生虫，与弓形虫具有许多特征，但明显不同。犬新孢子虫速殖子在体内和体外均可感染多种哺乳动物细胞。实现感染的机制很大程度上未知。最近的证据表明涉及一种受体-配体系统，其中一种或几种宿主细胞受体与一种或几种寄生虫配体结合。寄生虫细胞表面相关分子，例如最近鉴定出的Nc-p43抗原，是与这种物理相互作用有关的主要嫌疑人。在这项研究中表明，在寄生虫与亚凝集量的针对Nc-p43的亲和纯化抗体的温育下，对犬新孢子虫速殖子在体外Vero细胞单层侵袭的损害。用抗Nc-p43抗体进行的包埋后免疫金标记表明，Nc-p43不仅位于寄生虫细胞表面，而且位于致密的颗粒和变种中。还研究了Nc-p43在犬新孢子虫速殖子的细胞内增殖以及随后的寄生虫液泡成熟过程中的命运。

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