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首页> 外文期刊>Infection and immunity >Predominant recognition of the ESAT-6 protein in the first phase of interferon with Mycobacterium bovis in cattle.
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Predominant recognition of the ESAT-6 protein in the first phase of interferon with Mycobacterium bovis in cattle.

机译：在牛的牛分枝杆菌干扰素的第一阶段，主要识别ESAT-6蛋白。

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Tuberculosis continues to be a worldwide health problem for both humans and animals. The development of improved vaccines and diagnostic tests requires detailed understanding of the immune responses generated and the antigens recognized during the disease. This study examined the T-cell response which develops in cattle experimentally infected with Mycobacterium bovis. The first significant T-cell response was found 3 weeks after the onset of infection and was characterized by a pronounced gamma interferon (IFN-gamma) response from peripheral blood mononuclear cells directed to antigens in culture filtrates. Short-term culture filtrate (ST-CF) was separated into molecular mass fractions and screened for recognition by T cells from experimentally infected and field cases of bovine tuberculosis. Cattle in the early stages of experimental infection were characterized by strong IFN-gamma responses directed predominantly toward the lowest-mass (<10-kDa) fraction of ST-CF, but cattle in later stages of experimental infection (16 weeks postinfection) exhibited a broader recognition of antigens of various molecular masses. Field cases of bovine tuberculosis, in comparison, preferentially recognized low-mass antigens, characteristic of animals in the early stages of infection. The major T-cell target for this dominant IFN-gamma response was found to be the secreted antigen ESAT-6. This antigen was recognized strongly by the majority of field cases of bovine tuberculosis tested. As ESAT-6 is unique to pathogenic mycobacterial species, our study suggests that ESAT-6 is an antigen with major potential for vaccination against and specific diagnosis of bovine tuberculosis.
机译：结核病仍然是人类和动物的世界性健康问题。改进疫苗的开发和诊断测试需要对所产生的免疫反应和疾病期间识别的抗原的详细了解。这项研究检查了牛经分枝杆菌感染的牛的T细胞反应。感染开始后三周发现了第一个显着的T细胞反应，其特征是外周血单核细胞针对培养滤液中的抗原产生了明显的γ干扰素（IFN-γ）反应。将短期培养物滤液（ST-CF）分离为分子量部分，并筛选出经实验感染的牛结核病和田间病例的T细胞识别的T细胞。实验性感染早期的牛的特征是强烈的IFN-γ反应，主要针对ST-CF的最低质量（<10-kDa）部分，但实验性感染后期（感染后16周）的牛表现出较强的IFN-γ反应。更广泛地认识各种分子量的抗原。相比之下，牛结核病的野外病例优先识别出感染初期动物特有的低质量抗原。发现该主要IFN-γ应答的主要T细胞靶是分泌的抗原ESAT-6。该抗原被大多数测试的牛结核病现场病例强烈认可。由于ESAT-6是致病性分枝杆菌种所独有的，因此我们的研究表明ESAT-6是一种针对牛结核病进行疫苗接种和特异性诊断的主要抗原。

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