• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Assimilation of Xylose, Mannose, and Mannitol for Synthesis of Glucuronoxylomannan of Cryptococcus neoformansDetermined by 13C Nuclear Magnetic Resonance Spectroscopy
【24h】

Assimilation of Xylose, Mannose, and Mannitol for Synthesis of Glucuronoxylomannan of Cryptococcus neoformansDetermined by 13C Nuclear Magnetic Resonance Spectroscopy

机译：13 C核磁共振波谱法测定木糖，甘露糖和甘露醇的同化作用以合成新隐球菌的葡糖醛酸氧甘露聚糖

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cryptococcus neoformans NIH 409 was cultured in a defined medium containing d-[1-13C]xylose (Xyl), d-[1-13C]mannose (Man), ord-[1-13C]mannitol as the sole carbon source. The distribution of 13C in the Man, Xyl, glucuronic acid (GlcA), and O-acetyl constituents of native and de-O-acetylated glucuronoxylomannan (GXM) was determined by one-dimensional13C nuclear magnetic resonance spectroscopy. The carbon chain of Man was incorporated intact into GXM since13C was observed only in carbon 1 of Man, GlcA, and Xyl. The carbon chain of mannitol was incorporated intact into GXM since13C was observed only in carbons 1 and 6. This was expected since mannitol has an axis of symmetry. The carbon chain of Xyl was identified only in carbons 1 and 3 of Man, GlcA, and Xyl. This pattern of labeling is consistent with the assimilation of Xyl through the pentophosphate pathway.
机译：新型隐球菌将NIH 409在含有d- [1- 13 C]木糖（Xyl），d- [1- 13 C]甘露糖（Man），ord- [1- 13 C]甘露醇为唯一碳源。通过一维确定 13 C在天然和去O-乙酰化葡糖醛酸羟甘露聚糖（GXM）的Man，Xyl，葡萄糖醛酸（GlcA）和O-乙酰基成分中的分布13 C核磁共振波谱由于仅在Man，GlcA和Xyl的碳1中观察到 13 C，Man的碳链完整地并入了GXM。由于仅在碳1和6中观察到 13 C，甘露醇的碳链完整地结合到GXM中。这是可以预期的，因为甘露醇具有对称轴。 Xyl的碳链仅在Man，GlcA和Xyl的碳1和3中确定。这种标记方式与Xyl通过五磷酸途径的同化作用是一致的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号