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首页> 外文期刊>Infection and immunity >A Novel 62-Kilodalton Egg Antigen fromSchistosoma mansoni Induces a Potent CD4+ T Helper Cell Response in the C57BL/6 Mouse
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A Novel 62-Kilodalton Egg Antigen fromSchistosoma mansoni Induces a Potent CD4+ T Helper Cell Response in the C57BL/6 Mouse

机译：曼氏血吸虫的新型62千卵卵抗原在C57BL / 6小鼠中诱导有效的CD4 + T辅助细胞应答

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In infection with Schistosoma mansoni, hepatic granuloma formation is mediated by CD4+ T helper (Th) cells sensitized to schistosomal egg antigens. There is considerable variation among infected individuals with respect to both severity of disease and the T-cell response to egg antigens. In the BL/6 mouse, the egg granulomas are relatively small and the relevant sensitizing egg antigens are largely unknown. We investigated the CD4+ Th cell response of infected BL/6 mice to egg antigens fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found a prominent lymphoproliferative response to be directed against a 62-kDa component. With the aid of a specific T-cell hybridoma, 4E6, the 62-kDa antigen was isolated; following partial digestion with endoproteinase Glu-C, an internal amino acid sequence was found to be identical with one present in the enzyme phosphoenolpyruvate carboxykinase (PEPCK) of the organisms Caenorhabditis elegans and Treponema pallidum and to differ by one residue from PEPCK of various other species. In CD4+ Th cells from 7.5- 8.5-week-infected BL/6 mice, the purified 62-kDa molecule elicited a potent proliferative response which, based on cytokine analysis, was of a mixed Th-1 and Th-2 type. Our results reveal a novel egg antigen of particular prominence in the BL/6 mouse and suggest that the immune response in schistosomiasis is a product of sensitization to egg antigens that may vary considerably in immunogenicity from strain to strain.
机译：在曼氏血吸虫感染中，肝肉芽肿的形成是由对血吸虫卵抗原敏感的CD4 + T辅助（Th）细胞介导的。就疾病的严重程度和对卵抗原的T细胞反应而言，感染个体之间存在相当大的差异。在BL / 6小鼠中，卵肉芽肿相对较小，并且相关的致敏卵抗原很大程度上未知。我们研究了感染的BL / 6小鼠对十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的卵抗原的CD4 + Th细胞反应，发现针对62 kDa组分的显着淋巴细胞增生反应。借助于特定的T细胞杂交瘤4E6，分离出了62 kDa的抗原。用内蛋白酶Glu-C部分消化后，发现其内部氨基酸序列与秀丽隐杆线虫和苍白梅毒螺旋体的磷酸烯醇丙酮酸羧化激酶（PEPCK）中存在的氨基酸序列相同。并与其他各种物种的PEPCK区别一个残基。在感染了7.5- 8.5周的BL / 6小鼠的CD4 + Th细胞中，纯化的62 kDa分子引发了强增殖反应，基于细胞因子分析，它是混合的Th-1和Th-2型我们的结果揭示了在BL / 6小鼠中特别突出的新型卵抗原，并表明血吸虫病中的免疫应答是对卵抗原致敏的产物，卵抗原的免疫原性可能因菌株而异。

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