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首页> 外文期刊>Infection and immunity >A Novel Urease-Negative HelicobacterSpecies Associated with Colitis and Typhlitis in IL-10-Deficient Mice
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A Novel Urease-Negative HelicobacterSpecies Associated with Colitis and Typhlitis in IL-10-Deficient Mice

机译：IL-10缺乏症小鼠与结肠炎和伤寒相关的新型脲酶阴性幽门螺杆菌

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摘要

A spiral-shaped bacterium with bipolar, single-sheathed flagella was isolated from the intestines of IL-10 (interleukin-10)-deficient (IL-10?/?) mice with inflammatory bowel disease. The organism was microaerobic, grew at 37 and 42°C, and was oxidase and catalase positive but urease negative. On the basis of 16S rRNA gene sequence analysis and biochemical and phenotypic criteria, the organism is classified as a novel helicobacter. Cesarean section-rederived IL-10?/? mice without helicobacter infection did not have histological evidence of intestinal inflammation. However, helicobacter-free IL-10?/?, SCID/NCr, and A/JNCr mice experimentally inoculated with the novel urease-negativeHelicobacter sp. developed variable degrees of inflammation in the lower intestine, and in immunocompetent mice, the experimental infection was accompanied by a corresponding elevated immunoglobulin G antibody response to the novel Helicobacter sp. antigen. These data support other recent studies which demonstrate that multipleHelicobacter spp. in both naturally and experimentally infected mice can induce inflammatory bowel disease. The mouse model of helicobacter-associated intestinal inflammation should prove valuable in understanding how specific microbial antigens influence a complex disease process.
机译：从具有炎症性肠病的IL-10（白细胞介素10）缺陷型（IL-10 α/β）小鼠的肠中分离出具有双极单鞘鞭毛的螺旋形细菌。该生物是微需氧的，在37和42°C下生长，氧化酶和过氧化氢酶呈阳性，而脲酶呈阴性。根据16S rRNA基因序列分析以及生化和表型标准，该生物被归类为新型幽门螺杆菌。没有剖宫产的未感染幽门螺杆菌的IL-10 ？/？小鼠没有肠道炎症的组织学证据。然而，实验中用新型脲酶阴性的 Helicobacter sp接种了无幽门螺杆菌IL-10 ？/？，SCID / NCr和A / JNCr小鼠。在小肠内发生不同程度的炎症，在具有免疫能力的小鼠中，实验性感染伴随着对新型 Helicobacter sp的相应免疫球蛋白G抗体相应升高。抗原。这些数据支持其他最近的研究，这些研究证明了多个 Helicobacter spp。在自然和实验感染的小鼠中，均可诱发炎症性肠病。幽门螺杆菌相关的肠道炎症的小鼠模型应被证明对了解特定的微生物抗原如何影响复杂的疾病过程具有重要意义。

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